Multilocus enzyme electrophoresis analysis of rapidly-growing mycobacteria: an alternative tool for identification and typing

Highlights • Rapidly growing mycobacteria (RGM) are opportunistic pathogens associated with outbreaks. • Conventional identification of RGM species is laborious and uncertain. • Molecular techniques provide relevant information on diagnosis and clonality. • Multilocus enzyme electrophoresis (MLEE) w...

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Published inInternational journal of infectious diseases Vol. 42; no. C; pp. 11 - 16
Main Authors Caierão, Juliana, Paiva, José Augusto Cardoso Dias, Sampaio, Jorge Luiz Mello, Silva, Marlei Gomes da, Santos, Débora Ribeiro de Souza, Coelho, Fabrice Santana, Fonseca, Leila de Souza, Duarte, Rafael Silva, Armstrong, Derek T, Regua-Mangia, Adriana Hamond
Format Journal Article
LanguageEnglish
Published Canada Elsevier Ltd 01.01.2016
Elsevier
Subjects
Bacterial Typing Techniques - methods
Diversity
Electrophoresis
Female
Humans
Identification
Infectious Disease
Isoenzymes - analysis
MLEE
Molecular Typing
Mycobacterium chelonae - classification
Mycobacterium chelonae - enzymology
Mycobacterium fortuitum - classification
Mycobacterium fortuitum - enzymology
Proteomics - methods
Pulmonary/Respiratory
Rapidly growing mycobacteria
Typing
Identification
Typing
MLEE
Rapidly growing mycobacteria
Diversity
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Summary:Highlights • Rapidly growing mycobacteria (RGM) are opportunistic pathogens associated with outbreaks. • Conventional identification of RGM species is laborious and uncertain. • Molecular techniques provide relevant information on diagnosis and clonality. • Multilocus enzyme electrophoresis (MLEE) was found to be a powerful tool for the identification and typing of epidemic RGM species.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1201-9712
1878-3511
DOI:10.1016/j.ijid.2015.11.010