Development and Genome Sequencing of a Laboratory-Inbred Miniature Pig Facilitates Study of Human Diabetic Disease

• Published in: iScience Vol. 19; pp. 162 - 176
• Main Authors: Zhang, Li, Huang, Yuemeng, Wang, Meng, Guo, Yafen, Liang, Jing, Yang, Xiurong, Qi, Wenjing, Wu, Yanjun, Si, Jinglei, Zhu, Siran, Li, Zhe, Li, Ruiqiang, Shi, Chao, Wang, Shuo, Zhang, Qunjie, Tang, Zhonglin, Wang, Lixian, Li, Kui, Fei, Ji-Feng, Lan, Ganqiu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 27.09.2019; Elsevier
• Subjects: Biological Sciences; Gene Ontology; Genomic Analysis; Genomics; Omics; Sequence Analysis; Transcriptomics; Biological Sciences; Genomic Analysis; Transcriptomics; Genomics; Omics; Sequence Analysis; Gene Ontology
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2019.07.025

Pig has been proved to be a valuable large animal model used for research on diabetic disease. However, their translational value is limited given their distinct anatomy and physiology. For the last 30 years, we have been developing a laboratory Asian miniature pig inbred line (Bama miniature pig [BM]) from the primitive Bama xiang pig via long-term selective inbreeding. Here, we assembled a BM reference genome at full chromosome-scale resolution with a total length of 2.49 Gb. Comparative and evolutionary genomic analyses identified numerous variations between the BM and commercial pig (Duroc), particularly those in the genetic loci associated with the features advantageous to diabetes studies. Resequencing analyses revealed many differentiated gene loci associated with inbreeding and other selective forces. These together with transcriptome analyses of diabetic pig models provide a comprehensive genetic basis for resistance to diabetogenic environment, especially related to energy metabolism. [Display omitted] •Bama miniature pig (BM) is one of the pig lines with the highest inbreeding coefficient•This atlas is a report on the chromosome-level genome assembly of miniature pig•Genomic analyses revealed genetic basis underlying BM's advantages to study diabetes•Some lncRNAs and mRNAs may be linked to resistance to diabetogenic environment Biological Sciences; Gene Ontology; Genomic Analysis; Genomics; Omics; Sequence Analysis; Transcriptomics