Expression of the Human Fibroblast Interferon Gene in Escherichia coli

We applied the method of Guarente et al. [Guarente, L., Lauer, G., Roberts, T. M. & Ptashne, M. (1980) Cell 20, 543-553] to construct plasmids that direct expression in Escherichia coli of the human fibroblast interferon (F-IF) gene. Two plasmids were recovered. One directs efficient synthesis o...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 77; no. 9; pp. 5230 - 5233
Main Authors Taniguchi, Tadatsugu, Guarente, Leonard, Roberts, Thomas M., Kimelman, David, Douhan, John, Ptashne, Mark
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.09.1980
National Acad Sciences
Subjects
Antivirals
Bacteria
Base Sequence
Cloning, Molecular
DNA
DNA, Recombinant
Escherichia coli - genetics
Fibroblasts
Gene Expression Regulation
Genes
Genetic Vectors
Humans
Hybridity
Interferons
Interferons - biosynthesis
Interferons - genetics
Methods
Molecules
Nucleotide sequences
Operon
Plasmids
Vesicular stomatitis Indiana virus - drug effects
Online AccessGet full text

Cover

More Information
Summary:We applied the method of Guarente et al. [Guarente, L., Lauer, G., Roberts, T. M. & Ptashne, M. (1980) Cell 20, 543-553] to construct plasmids that direct expression in Escherichia coli of the human fibroblast interferon (F-IF) gene. Two plasmids were recovered. One directs efficient synthesis of a protein whose primary sequence is that of pre-F-IF and the other, that of mature F-IF. Extracts of bacteria synthesizing mature F-IF display antiviral activity characteristic of human F-IF. This activity is lower than that expected from the differential rate of synthesis of the protein. We have detected no such activity in extracts of bacteria synthesizing pre-F-IF.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.9.5230