A novel application of the buccal micronucleus cytome assay in oral lichen planus: A pilot study

• Published in: Archives of oral biology Vol. 56; no. 10; pp. 1148 - 1153
• Main Authors: Sanchez-Siles, M., Ros-Llor, I., Camacho-Alonso, F., Lopez-Jornet, P.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2011
• Subjects: Advanced Basic Science; Age; Basal cells; Buccal micronucleus cytome assay; Buds; Case-Control Studies; Cell Death; Cell Differentiation; Cell Nucleus - classification; Cell Nucleus - ultrastructure; Cell Nucleus Shape; Cell Proliferation; Centrifuges; Cheek; Chromatin; Chromatin - ultrastructure; Cytodiagnosis - instrumentation; Dentistry; DNA Damage; Female; Fluorescent Dyes; Humans; Indoles; Lichen planus; Lichen Planus, Oral - pathology; Male; Malignancy; Micronuclei; Micronuclei, Chromosome-Defective - classification; Micronucleus Tests - methods; Microscopes; Microscopy, Fluorescence; Middle Aged; Mouth Mucosa - ultrastructure; Oral cancer; Oral lichen planus; Pilot Projects; Slide preparation; Transformation; Oral lichen planus; Oral cancer; Buccal micronucleus cytome assay
• ISSN: 0003-9969; 1879-1506; 1879-1506
• DOI: 10.1016/j.archoralbio.2011.02.019

The most important complication of oral lichen planus is malignancy transformation. The aim of this study was to assess cellular and nuclear morphology in a group of patients with oral lichen planus measured by means of buccal micronucleus cytome assay. This study included thirty patients with a clinicopathological diagnosis of oral lichen planus (all with atrophic–erosive clinical forms of OLP) and thirty healthy control subjects. Both samples were similar in age and gender. The buccal micronucleus cytome assay protocol consisted of: cell collection from both cheeks with a cytobrush; cell centrifuge; slide preparation, fixation and staining followed by fluorescent microscope analysis. 2×106 exfoliated cells were screened for nuclear abnormalities: micronuclei, nuclear buds, binucleation, basal and differentiated cells, condensed chromatin, karyorrhectic cells, pyknosis and karyolytic cells. Patients with oral lichen planus showed significantly higher frequencies of micronuclei (p<0.001), nuclear buds (p<0.001), binucleated cells (p<0.021) than the control group. This method is an easy way for clinicians to assess DNA damage, proliferative potential of basal cells and cell death in buccal cells in cases of oral lichen planus.