Obtaining Human Breast Adipose Cells for Breast Cancer Cell Co-culture Studies

• Published in: STAR protocols Vol. 1; no. 3; p. 100197
• Main Authors: Picon-Ruiz, Manuel, Marchal, Juan A., Slingerland, Joyce M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.12.2020; Elsevier
• Subjects: Adipocytes - cytology; Adipose Tissue - cytology; Adipose Tissue - pathology; Breast - pathology; Breast Neoplasms - pathology; Cancer; Cell culture; Cell Differentiation; Cell isolation; Cell Proliferation; Coculture Techniques - methods; Female; Humans; Protocol; Specimen Handling - methods; Stem Cells - cytology; Cell culture; Cell Differentiation; Cell isolation; Cancer
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2020.100197

Primary human breast cancers invade surrounding fat and contact adipocytes, inflammatory infiltrates, and fibrous stroma. This tissue niche influences breast tumor progression. Here, we present a protocol to enable the in vitro study of the complex interactions that occur between breast cancer cells and adipose cells. We describe how to obtain different adipose cell populations, including adipose-derived stem cells, immature adipocytes, and mature adipocytes, from human breast fat tissue and detail the application for co-culture assays with breast cancer cells. For complete details on the use and execution of this protocol, please refer to Picon-Ruiz et al. (2016) and Qureshi et al. (2020). [Display omitted] •Obtaining different adipose cell populations from human breast fat samples•Isolation of mature adipocytes, immature adipocytes, and hASC•Co-culture of breast cancer cells with isolated primary human breast fat populations Primary human breast cancers invade surrounding fat and contact adipocytes, inflammatory infiltrates, and fibrous stroma. This tissue niche influences breast tumor progression. Here, we present a protocol to enable the in vitro study of the complex interactions that occur between breast cancer cells and adipose cells. We describe how to obtain different adipose cell populations, including adipose-derived stem cells, immature adipocytes, and mature adipocytes, from human breast fat tissue and detail the application for co-culture assays with breast cancer cells.