Multi-Omic Analyses of Growth Cones at Different Developmental Stages Provides Insight into Pathways in Adult Neuroregeneration

Growth cones (GCs) are structures associated with growing neurons. GC membrane expansion, which necessitates protein-lipid interactions, is critical to axonal elongation in development and in adult neuritogenesis. We present a multi-omic analysis that integrates proteomics and lipidomics data for th...

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Published iniScience Vol. 23; no. 2; p. 100836
Main Authors Chauhan, Muhammad Zain, Arcuri, Jennifer, Park, Kevin K., Zafar, Maroof Khan, Fatmi, Rabeet, Hackam, Abigail S., Yin, Yuqin, Benowitz, Larry, Goldberg, Jeffrey L., Samarah, Mohammad, Bhattacharya, Sanjoy K.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 21.02.2020
Elsevier
Subjects
Biological Sciences
Lipidomics
Omics
Proteomics
Systems Biology
Biological Sciences
Lipidomics
Systems Biology
Omics
Proteomics
Online AccessGet full text
ISSN2589-0042
2589-0042
DOI10.1016/j.isci.2020.100836

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Summary:Growth cones (GCs) are structures associated with growing neurons. GC membrane expansion, which necessitates protein-lipid interactions, is critical to axonal elongation in development and in adult neuritogenesis. We present a multi-omic analysis that integrates proteomics and lipidomics data for the identification of GC pathways, cell phenotypes, and lipid-protein interactions, with an analytic platform to facilitate the visualization of these data. We combine lipidomic data from GC and adult axonal regeneration following optic nerve crush. Our results reveal significant molecular variability in GCs across developmental ages that aligns with the upregulation and downregulation of lipid metabolic processes and correlates with distinct changes in the lipid composition of GC plasmalemma. We find that these processes also define the transition into a growth-permissive state in the adult central nervous system. The insight derived from these analyses will aid in promoting adult regeneration and functional innervation in devastating neurodegenerative diseases. [Display omitted] •Simultaneous proteomics and lipidomics analyses of developmental growth cones•Combined multi-omics analyses of regenerating optic nerves and growth cones•Integrating protein-protein with protein-lipid interactions in growth cones Biological Sciences; Systems Biology; Omics; Proteomics; Lipidomics
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ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2020.100836