Nectin-4 Interactions Govern Measles Virus Virulence in a New Model of Pathogenesis, the Squirrel Monkey (Saimiri sciureus)

• Published in: Journal of virology Vol. 91; no. 11; pp. 129 - 136
• Main Authors: Delpeut, Sébastien, Sawatsky, Bevan, Wong, Xiao-Xiang, Frenzke, Marie, Cattaneo, Roberto, von Messling, Veronika
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.06.2017
• Subjects: Animal genetics; Animals; Cell Adhesion Molecules - metabolism; Cynomolgus; Epithelial Cells - virology; Genetics; Green Fluorescent Proteins; Humans; Leukocytes, Mononuclear - chemistry; Leukocytes, Mononuclear - virology; Life Sciences; Macaca fascicularis; Measles - virology; Measles virus; Measles virus - pathogenicity; Measles virus - physiology; Microbiology and Parasitology; Models, Animal; Nectins; Pathogenesis and Immunity; Saimiri; Saimiri sciureus; Viral Load; Virology; Virulence; pathogenesis; primate models; cellular receptors; immune suppression; measles virus
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/jvi.02490-16

In addition to humans, only certain nonhuman primates are naturally susceptible to measles virus (MeV) infection. Disease severity is species dependent, ranging from mild to moderate for macaques to severe and even lethal for certain New World monkey species. To investigate if squirrel monkeys ( ), which are reported to develop a course of disease similar to humans, may be better suited than macaques for the identification of virulence determinants or the evaluation of therapeutics, we infected them with a green fluorescent protein-expressing MeV. Compared to cynomolgus macaques ( ) infected with the same virus, the squirrel monkeys developed more-severe immunosuppression, higher viral load, and a broader range of clinical signs typical for measles. In contrast, infection with an MeV unable to interact with the epithelial receptor nectin-4, while causing immunosuppression, resulted in only a mild and transient rash and a short-lived elevation of the body temperature. Similar titers of the wild-type and nectin-4-blind MeV were detected in peripheral blood mononuclear cells and lymph node homogenates, but only the wild-type virus was found in tracheal lavage fluids and urine. Thus, our study demonstrates the importance of MeV interactions with nectin-4 for clinical disease in the new and better-performing model of measles pathogenesis. The characterization of mechanisms underlying measles virus clinical disease has been hampered by the lack of an animal model that reproduces the course of disease seen in human patients. Here, we report that infection of squirrel monkeys ( ) fulfills these requirements. Comparative infection with wild-type and epithelial cell receptor-blind viruses demonstrated the importance of epithelial cell infection for clinical disease, highlighting the spread to epithelia as an attractive target for therapeutic strategies.