Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model

• Published in: Pharmaceutics Vol. 16; no. 2; p. 174
• Main Authors: Moura, Michely Laiany Vieira, de Menezes, Ag-Anne Pereira Melo, de Oliveira Filho, José Williams Gomes, do Nascimento, Maria Luiza Lima Barreto, Dos Reis, Antonielly Campinho, Ribeiro, Alessandra Braga, da Silva, Felipe Cavalcanti Carneiro, Nunes, Adriana Maria Viana, Rolim, Hercília Maria Lins, de Carvalho Melo Cavalcante, Ana Amélia, Sousa, João Marcelo de Castro E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.01.2024; MDPI
• Subjects: Accountants; Analysis; Animals; Banking industry; Breast cancer; Cancer therapies; Chemotherapy; Cyclophosphamide; Cytotoxicity; DNA damage; Drug delivery systems; Drug dosages; Efficiency; Ethylenediaminetetraacetic acid; fungal metabolites; Hematology; Hemoglobin; Liver; Mice; nanotechnology; Olive oil; Toxicity; Canada; United States; nanotechnology; breast cancer; fungal metabolites; cytotoxicity
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics16020174

The study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). virgin females were divided into six groups treated with (1) olive oil (10 mL/kg); (2) 7,12-DMBA (6 mg/kg); (3) citrinin, CIT (2 mg/kg), (4) cyclophosphamide, CPA (25 mg/kg), (5) liposomal citrinin, LP-CIT (2 μg/kg), and (6) LP-CIT (6 µg/kg). Metabolic, behavioral, hematological, biochemical, histopathological, and toxicogenetic tests were performed. DMBA and cyclophosphamide induced behavioral changes, not observed for free and liposomal citrinin. No hematological or biochemical changes were observed for LP-CIT. However, free citrinin reduced monocytes and caused hepatotoxicity. During treatment, significant differences were observed regarding the weight of the right and left breasts treated with DMBA compared to negative controls. Treatment with CPA, CIT, and LP-CIT reduced the weight of both breasts, with better results for liposomal citrinin. Furthermore, CPA, CIT, and LP-CIT presented genotoxic effects for tumor, blood, bone marrow, and liver cells, although less DNA damage was observed for LP-CIT compared to CIT and CPA. Healthy cell damage induced by LP-CIT was repaired during treatment, unlike CPA, which caused clastogenic effects. Thus, LP-CIT showed advantages for its use as a model of nanosystems for antitumor studies.