Development of Anti-atypical Aeromonas salmonicida Monoclonal Antibodies for Diagnosis of “New Ulcer Disease” in Koi Carp

To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pat...

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Bibliographic Details
Published inFish Pathology Vol. 53; no. 1; pp. 36 - 39
Main Authors Somamoto, Tomonori, Maruyama, Shinpei, Nagasawa, Takahiro, Nakao, Miki, Sato, Atsushi, Hatta, Hajime, Sato, Mitsuru, Murakami-Yamaguchi, Yukie, Kizu-Mori, Kumiko, Hirakawa, Yuki, Narita, Hiroshi
Format Journal Article
LanguageEnglish
Published Tokyo The Japanese Society of Fish Pathology 2018
Japan Science and Technology Agency
Subjects
Aeromonas salmonicida
Antibodies
atypical Aeromonas salmonicida
Bacteria
Fish
Freshwater fishes
koi carp
Marine fishes
Microbiological strains
Monoclonal antibodies
monoclonal antibody
new ulcer disease
Pathogenic bacteria
Ulcers
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Summary:To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromonads.
ISSN:0388-788X
1881-7335
DOI:10.3147/jsfp.53.36