Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate

Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 107; no. 15; pp. 6946 - 6951
Main Authors Silverman, Sanford J, Petti, Allegra A, Slavov, Nikolai, Parsons, Lance, Briehof, Ryan, Thiberge, Stephan Y, Zenklusen, Daniel, Gandhi, Saumil J, Larson, Daniel R, Singer, Robert H, Botstein, David
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 13.04.2010
National Acad Sciences
Subjects
Biological Sciences
cell culture
Cell cycle
Cell Division
Cell growth
Cells
Cellular metabolism
Correlations
Cultured cells
Data ranges
Energy Metabolism
fluorescence in situ hybridization
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Fungal
Genes, Fungal
Genetics
Glucose
Glucose - metabolism
In Situ Hybridization, Fluorescence
Messenger RNA
metabolism
microbial physiology
Models, Biological
nutrient availability
nutrient deficiencies
Oscillometry
Phosphates
Phosphates - metabolism
phosphorus
Qualitative research
Ribonucleic acid
RNA
RNA, Messenger - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Yeast
Yeasts
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Summary:Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells drawn from unsynchronized cultures growing exponentially in chemostats. Gene-expression data from synchronized cultures were used to predict coincident appearance of mRNAs from pairs of genes in the unsynchronized cells. Quantitative analysis of the FISH results shows that individual unsynchronized cells growing slowly because of glucose limitation or phosphate limitation show the predicted oscillations. We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.
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Author contributions: S.J.S., S.Y.T., and D.B. designed research; S.J.S., R.B., and D.Z. performed research; S.J.S., A.A.P., N.S., L.P., S.Y.T., D.Z., S.J.G., D.R.L., and R.H.S. contributed new reagents/analytic tools; A.A.P., N.S., L.P., and D.B. analyzed data; and S.J.S., A.A.P., N.S., and D.B. wrote the paper.
1A.A.P. and N.S. contributed equally to this work.
Contributed by David Botstein, February 28, 2010 (sent for review January 25, 2010)
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1002422107