Strategy for Screening of Antioxidant Compounds from Two Ulmaceae Species Based on Liquid Chromatography-Mass Spectrometry

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 7; p. 1830
• Main Authors: Won, Joong Yeun, Son, Su Young, Lee, Sunmin, Singh, Digar, Lee, Sarah, Lee, Jong Seok, Lee, Choong Hwan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.07.2018; MDPI
• Subjects: antioxidant activity; Antioxidants; Aphananthe aspera; Biological activity; Cancer; Chromatography; Discriminant analysis; Flavonoids; Flowers & plants; Food; Glucosides; High performance liquid chromatography; Kaempferol; LC-MS; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolism; metabolite profiling; Metabolites; Metabolomics; NMR; Nuclear magnetic resonance; Phylogenetics; Phytochemicals; Plant extracts; preparative HPLC combination; Principal components analysis; Quercetin; R&D; Research & development; Scientific imaging; Solid phases; Spectroscopy; Statistical analysis; Sulfonic acid; Ulmaceae; Zelkova serrata; preparative HPLC combination; Aphananthe aspera; LC-MS; antioxidant activity; metabolite profiling; Zelkova serrata
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23071830

Liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics implies that annotated metabolites can serve as potential markers of the associated bioactivities of plant extracts. Firstly, we selected and (Family: Ulmaceae) from 16 Korean plant species based on their distinct principal component analysis (PCA) patterns in LC-MS datasets and antioxidant activity assays. Further, we chose 40% solid-phase extraction (SPE) extracts of the two species displaying the highest antioxidant activities coupled with distinct PCA patterns. Examining the metabolite compositions of the 40% SPE extracts, we observed relatively higher abundances of quercetin, kaempferol, and isorhamnetin -glucosides for , whereas quercetin, isorhamnetin -glucuronides, and procyanidin dimer were relatively higher in . These metabolites were clearly distinguished in pathway map and displayed strong positive correlations with antioxidant activity. Further, we performed preparative high-performance liquid chromatography (prep-HPLC) analysis coupled with the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assay to validate their functional correlations. As a result, quercetin -sophoroside was determined as the main antioxidant in , while isorhamnetin -glucuronide and procyanidin dimer were the primary antioxidants in . The current study suggests that the LC-MS-based untargeted metabolomics strategy can be used to illuminate subtle metabolic disparities as well as compounds associated with bioactivities.