Xpert MTB/RIF and GenoType MTBDRplus assays for the rapid diagnosis of bone and joint tuberculosis

• Published in: International journal of infectious diseases Vol. 36; no. C; pp. 27 - 30
• Main Authors: Gu, Yunting, Wang, Guirong, Dong, Weijie, Li, Yunxu, Ma, Yifeng, Shang, Yuanyuan, Qin, Shibing, Huang, Hairong
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.07.2015; Elsevier
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Bone and joint tuberculosis; Drug Resistance, Bacterial; Female; Genotype; GenoType MTBDRplus; Humans; Infectious Disease; Isoniazid - pharmacology; Male; Microbial Sensitivity Tests; Middle Aged; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Polymerase Chain Reaction; Pulmonary/Respiratory; Rifampin - pharmacology; Sensitivity and Specificity; Tuberculosis, Osteoarticular - diagnosis; Tuberculosis, Osteoarticular - microbiology; Xpert MTB/RIF; Young Adult; GenoType MTBDRplus; Bone and joint tuberculosis; Xpert MTB/RIF
• ISSN: 1201-9712; 1878-3511
• DOI: 10.1016/j.ijid.2015.05.014

Highlights • Xpert MTB/RIF (Xpert) and GenoType MTBDRplus (MTBDR) assays were evaluated rigorously for the rapid diagnosis of bone and joint tuberculosis (BJTB). • The sensitivities of Xpert and MTBDR were 82% and 72%, respectively, and the specificities were both 100%. • Xpert was 100% concordant with drug susceptibility testing for the detection of rifampicin resistance. • The sensitivity of MTBDR for the detection of rifampicin and isoniazid resistance was 83.3% and 85.7%, respectively. • With their high sensitivities and short turnaround times, Xpert and MTBDR are feasible for the diagnosis of BJTB.