Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats

• Published in: Journal of Veterinary Medical Science Vol. 82; no. 11; pp. 1662 - 1665
• Main Authors: GAO, Yang, GUO, Huanping, MOUMOUNI, Paul Franck Adjou, LIU, Mingming, LI, Jixu, EFSTRATIOU, Artemis, GALON, Eloiza May, YBANEZ, Rochelle Haidee, MASATANI, Tatsunori, SOMA, Takehisa, XUAN, Xuenan
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2020; The Japanese Society of Veterinary Science
• Subjects: Animals; Antibodies, Protozoan; Antigens, Protozoan; cat; Cat Diseases - diagnosis; Cats; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Mice; Parasitology; Protozoan Proteins - genetics; SAG1-related sequence 2 of Toxoplasma gondii; Sensitivity and Specificity; serodiagnosis; Serologic Tests - veterinary; Toxoplasma; toxoplasmosis; Toxoplasmosis, Animal - diagnosis; serodiagnosis; SAG1-related sequence 2 of Toxoplasma gondii; toxoplasmosis; cat; enzyme-linked immunosorbent assay
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.20-0231

An enzyme-linked immunosorbent assay (ELISA) based on recombinant SAG1-related sequence 2 of Toxoplasma gondii (rTgSRS2) was developed to detect toxoplasmosis in cats. The specificity and sensitivity of rTgSRS2 ELISA were confirmed using a series of serum samples from T. gondii-experimentally infected mice. A total of 76 field samples from cats were examined by the developed ELISA. The rTgSRS2 ELISA showed a good diagnostic performance characterized by high concordance (88.16) and kappa value (0.76) with latex agglutination test (LAT). The sensitivity and specificity of the test were 92.68% and 82.86%, respectively. These results suggest that the ELISA based on rTgSRS2 could be a useful tool for serodiagnosis of T. gondii infection in cats.