Eye extract improves cell migration out of lymphoid organ explants of L. vannamei and viability of the primary cell cultures

Since no cell line from shrimp has been established up till now, an optimization of the primary cell culture protocol is necessary. In this context, the effect of extracts (supernatant of a 1:50 (w/v) suspension) from different shrimp organs (muscle, brain, ganglia, eyestalk, ovary, and eye) on the...

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Published inIn vitro cellular & developmental biology. Animal Vol. 51; no. 7; pp. 651 - 654
Main Authors Li, Wenfeng, Van Tuan, Vo, Van Thuong, Khuong, Bossier, Peter, Nauwynck, Hans
Format Journal Article
LanguageEnglish
Published New York Springer US 01.08.2015
Springer Science & Business Media LLC
Society for In Vitro Biology
Subjects
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
brain
Cell Biology
Cell Culture
cell movement
Cell Movement - drug effects
Cell Survival - drug effects
Decapoda
Developmental Biology
explants
Eye - chemistry
eyes
Female
ganglia
Life Sciences
Litopenaeus vannamei
muscles
Muscles - chemistry
Organ Culture Techniques - methods
Ovary - chemistry
Penaeidae - chemistry
Penaeidae - cytology
Primary Cell Culture - methods
shrimp
Stem Cells
Tissue Extracts - pharmacology
viability
Eye extract
Cell culture
Lymphoid organ
Ovary extract
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Summary:Since no cell line from shrimp has been established up till now, an optimization of the primary cell culture protocol is necessary. In this context, the effect of extracts (supernatant of a 1:50 (w/v) suspension) from different shrimp organs (muscle, brain, ganglia, eyestalk, ovary, and eye) on the performance of primary lymphoid cell cultures was evaluated. Ten percent of eye extract and 3% of ovary extract enhanced maximally the migration and survival of cells of the lymphoid organ of Litopenaeus vannamei significantly at 48, 96, and 144 h post seeding. Extracts from the eyestalk (10%), muscle (10%), and brain (1%) significantly promoted the migration and survival of cells at 48 and 96 h post seeding but not anymore at 144 h post seeding. In conclusion, it may be advised to add 10% of eye extract or 3% of ovary extract to cells for the maximal health of primary cell cultures from the lymphoid organ of L. vannamei.
Bibliography:http://dx.doi.org/10.1007/s11626-015-9882-2
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ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-015-9882-2