Responses of Wild-Type and Resistant Strains of the Hyperthermophilic Bacterium Thermotoga maritima to Chloramphenicol Challenge

Transcriptomes and growth physiologies of the hyperthermophile Thermotoga maritima and an antibiotic-resistant spontaneous mutant were compared prior to and following exposure to chloramphenicol. While the wild-type response was similar to that of mesophilic bacteria, reduced susceptibility of the m...

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Published inApplied and Environmental Microbiology Vol. 73; no. 15; pp. 5058 - 5065
Main Authors Montero, Clemente I, Johnson, Matthew R, Chou, Chung-Jung, Conners, Shannon B, Geouge, Sarah G, Tachdjian, Sabrina, Nichols, Jason D, Kelly, Robert M
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.08.2007
Subjects
Anti-Bacterial Agents - pharmacology
Antibiotics
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence
Biological and medical sciences
Chloramphenicol - pharmacology
Drug resistance
Drug Resistance, Bacterial - genetics
Evolutionary and Genomic Microbiology
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Hot Temperature
Microbial Sensitivity Tests
Microbiology
Molecular Sequence Data
Mutation
Oligonucleotide Array Sequence Analysis
Point Mutation
Proteome
Ribozymes
RNA, Ribosomal, 23S - genetics
Thermotoga maritima
Thermotoga maritima - drug effects
Thermotoga maritima - genetics
Thermotoga maritima - growth & development
Transcription, Genetic
Resistance
Bacteria
Chloramphenicol
Hyperthermophily
Thermotoga maritima
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Summary:Transcriptomes and growth physiologies of the hyperthermophile Thermotoga maritima and an antibiotic-resistant spontaneous mutant were compared prior to and following exposure to chloramphenicol. While the wild-type response was similar to that of mesophilic bacteria, reduced susceptibility of the mutant was attributed to five mutations in 23S rRNA and phenotypic preconditioning to chloramphenicol.
Bibliography:http://aem.asm.org/contents-by-date.0.shtml
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Corresponding author. Mailing address: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905. Phone: (919) 515-6396. Fax: (919) 515-3465. E-mail: rmkelly@eos.ncsu.edu
Present address: Wyeth Pharmaceuticals, Sanford, NC 27330.
Present address: National Institutes of Health, Bethesda, MD 20892.
Present address: SAS Corporation, Cary, NC 27513.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.00453-07