In vitro immuno-stimulatory and anticancer activities of Oroxylum indicum (L.) Kurz.: An evidence for substitution of aerial parts for conservation

• Published in: Journal of ayurveda and integrative medicine Vol. 13; no. 2; p. 100523
• Main Authors: Rai, Deeksha, Aswatha Ram, H.N., Neeraj Patel, K., Babu, U.V., Sharath Kumar, L.M., Kannan, R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier B.V 01.04.2022; Elsevier
• Subjects: Leaves; Original; Oroxylum indicum (L.) Kurz; Roots; Stems; Oroxylum indicum (L.) Kurz; Leaves; Stems; Roots
• ISSN: 0975-9476; 0976-2809
• DOI: 10.1016/j.jaim.2021.09.001

In Ayurveda, “Dashamoolarishta” is one of the important composite herbal formulations. Mainly, the root and root bark of Oroxylum indicum are used as one of the ingredients in its preparation. This leads to over exploitation of medicinal plants owing, to excessive demand due to population expansion and its perceived importance in traditional herbal remedies. For the conservation of biodiversity, the present investigation had an objective to prepare the extracts of different parts of O. indicum plant and to, compare the chemo-profiles as well as to study the biological activities of the prepared extracts. Hydro-alcoholic (HA) and aqueous (Aq) extracts of various plant parts were prepared and chemical investigation was done with the help of (LC-MS/MS). Further, in vitro biological activities such as immuno-stimulation (IS) using a cytokine bioassay in RAW264.7 and in vitro anticancer in TNF-α ELISA in THP-1 cells were studied. The mass spectral profile of the plant revealed the presence of markers such as oroxylin A and chrysin in HA and Aq extracts of stem, leaf, bark and root. Cytokine release and TNF-α secretion was observed in both hydro-alcoholic and aqueous extracts. Based on the results from the present study, it can be concluded that it is possible to replace the roots and the bark of O. indicum with the stem of young plants and leaves. It paves a way for the conserving the medicinal plants without uprooting and extinguishing the whole plant.