Phylogenetic network analysis applied to pig gut microbiota identifies an ecosystem structure linked with growth traits

• Published in: The ISME Journal Vol. 10; no. 12; pp. 2973 - 2977
• Main Authors: Ramayo-Caldas, Yuliaxis, Mach, Nuria, Lepage, Patricia, Levenez, Florence, Denis, Catherine, Lemonnier, Gaetan, Leplat, Jean-Jacques, Billon, Yvon, Berri, Mustapha, Doré, Jöel, Rogel-Gaillard, Claire, Estellé, Jordi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2016; Nature Publishing Group
• Subjects: 38/22; 38/23; 38/77; 631/158/855; 631/208/191; Animal biology; Animal production; Animals; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Biomedical and Life Sciences; Biota; Body weight; Cluster Analysis; Ecology; Ecosystem structure; Evolutionary Biology; Feces - microbiology; Gastrointestinal Microbiome; Humans; Life Sciences; Microbial activity; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Phylogeny; Prevotella; RNA, Ribosomal, 16S - genetics; Ruminococcus; Short Communication; Swine; Swine - growth & development; Swine - metabolism; Swine - microbiology
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/ismej.2016.77

The ecological interactions within the gut microbial communities are complex and far from being fully understood. Here we report the first study that aims at defining the interaction network of the gut microbiota in pigs and comparing it with the enterotype-like clustering analysis. Fecal microbiota of 518 healthy piglets was characterized by 16S ribosomal RNA gene sequencing. Two networks were constructed at the genus and operational taxonomic unit levels. Within-network interactions mirrored the human gut microbiota relationships, with a strong co-exclusion between Prevotella and Ruminococcus genera, and were consistent with the two enterotype-like clusters identified in the pig microbiota. Remarkably, the cluster classification of the individuals was significantly associated with the body weight at 60 days of age ( P =0.005) and average daily gain ( P =0.027). To the best of our knowledge, this is the first study to provide an integrated overview of the porcine gut microbiota that suggests a conservation of the ecological community interactions and functional architecture between humans and pig. Moreover, we show that the microbial ecosystems and porcine growth traits are linked, which allows us to foresee that the enterotype concept may have an important role in the animal production industry.