Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia

In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia ("La Balsa"). RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bayesian inference usin...

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Published inRevista da Sociedade Brasileira de Medicina Tropical Vol. 52; p. e20190060
Main Authors Hoyos-López, Richard, Atencia-Pineda, Maria Claudia, Gallego-Gómez, Juan Carlos
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil Sociedade Brasileira de Medicina Tropical - SBMT 01.01.2019
Sociedade Brasileira de Medicina Tropical (SBMT)
Subjects
Aedes - virology
Aedes aegypti
Animals
Bayes Theorem
Colombia
Colombia - epidemiology
Dengue - epidemiology
Dengue - virology
Dengue Virus - classification
Dengue Virus - genetics
Dengue Virus - isolation & purification
DENV-2
Genotypes
Mosquito Vectors - virology
Phylogenetic
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Serogroup
Serotyping
Severe Dengue
TROPICAL MEDICINE
Colombia
Phylogenetic
Colombia
Aedes aegypti
DENV-2
Genotypes
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Summary:In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia ("La Balsa"). RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bayesian inference using reference DENV-2 sequences were performed. Twelve pools that tested positive for DENV-2 were characterized based on the C-PrM region and grouped under the Asian/American clade. This study is the first to report the DENV-2 Asian-American subtype in a rural area of Cordoba region, which is associated with severe dengue and local epidemics.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0037-8682
1678-9849
1678-9849
DOI:10.1590/0037-8682-0060-2019