Mitochondrial DNA content affects the fertilizability of human oocytes

Mitochondrial DNA content varies considerably in oocytes, even when collected from the same patient. In the present study, real-time quantitative polymerase chain reaction analysis of 113 unfertilized oocytes obtained from 43 patients revealed an average of 193 000 (range: 20 000 to 598 000) mitocho...

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Published inMolecular human reproduction Vol. 7; no. 5; pp. 425 - 429
Main Authors Reynier, P., May-Panloup, P., Chrétien, M-F., Morgan, C.J., Jean, M., Savagner, F., Barrière, P., Malthièry, Y.
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 01.05.2001
Oxford Publishing Limited (England)
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Summary:Mitochondrial DNA content varies considerably in oocytes, even when collected from the same patient. In the present study, real-time quantitative polymerase chain reaction analysis of 113 unfertilized oocytes obtained from 43 patients revealed an average of 193 000 (range: 20 000 to 598 000) mitochondrial genomes per cell. We compared several groups of oocytes to investigate the relationship between mitochondrial DNA content and fertilizability. The average mitochondrial DNA copy number was significantly lower in cohorts suffering from fertilization failure compared to cohorts with a normal rate of fertilization. In addition, the mitochondrial copy number of oocytes from patients with fertilization failure due to unknown causes was significantly lower than that of oocytes from patients in which IVF failure was due mainly to a severe sperm defect. The lower mtDNA copy number could be due to defective cytoplasmic maturation of oocytes. We conclude that low mitochondrial DNA content, due to inadequate mitochondrial biogenesis or cytoplasmic maturation, may adversely affect oocyte fertilizability.
Bibliography:istex:11FC5B8669A8766B581E535630038A969E04A245
local:0070425
PII:1460-2407
ark:/67375/HXZ-H18LW6D5-4
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1360-9947
1460-2407
DOI:10.1093/molehr/7.5.425