Osteocyte Wnt/β-catenin pathway activation upon mechanical loading is altered in ovariectomized mice

• Published in: Bone Reports Vol. 15; p. 101129
• Main Authors: Jackson, Erica, Lara-Castillo, Nuria, Akhter, Mohammed P., Dallas, Mark, Scott, JoAnna M., Ganesh, Thiagarajan, Johnson, Mark L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2021; Elsevier
• Subjects: Full Length; Mechanical loading; Osteocytes; Ovariectomy; β-Catenin signaling; Osteocytes; Ovariectomy; β-Catenin signaling; Mechanical loading
• ISSN: 2352-1872; 2352-1872
• DOI: 10.1016/j.bonr.2021.101129

Estrogen levels decline in both sexes with age, but more dramatically in females. Activation of the Wnt/β-catenin signaling pathway is central to the regulation of bone mass accrual and maintenance and in response to mechanical loading. Using the ovariectomized mouse model we examined the effect of estrogen loss on the osteocyte's ability to activate the Wnt/β-catenin pathway following mechanical loading. Female TOPGAL mice underwent ovariectomy (OVX) (n = 10) or sham surgery (n = 10) at 16 weeks of age. Four weeks post-surgery, a single loading session (global strain of 2200 με for 100 cycles at 2 Hz) was performed on the right forearm with the left as a non-loaded control. Mice (n = 5) were sacrificed at 1 or 24 hr post-load. Ulnae were stained for β-catenin activation, femurs were used for μCT and 3-pt bending/biomechanical testing, and tibiae were used for histology analysis and to determine osteocyte lacunar size using SEM and high resolution micro-XCT. A 2.2-fold increase in β-catenin signaling activation was observed 24 hr post-load in the Sham group but did not occur in the OVX group. The OVX group versus control had significant losses (p < 0.05) in trabecular BMD (−8%), BV/TV (−35%) and thickness (−23%), along with cortical thickness (−6%) and periosteal perimeter (−4%). The OVX group had significantly higher trabecular bone osteoclast numbers (63%), OCS/BS (77%) and N.OC/BPm (94%) and a significant decrease in osteoblast number (53%), OBS/BS (37%) and N.OB/BPm (40%) compared to the sham group (p < 0.05). Cortical bone lacunar number/lacunar volume and bone biomechanical properties did not change between groups. Given that the ulna is a cortical bone loading model and the lack of changes in osteocyte lacunar number/volume in cortical bone, which would alter strains experienced by osteocytes, these data suggest the absence of estrogen resulted in intrinsic changes in the ability of the osteocyte to respond to mechanical load, rather than changes in the biomechanical and architectural properties of bone. •In vivo mechanical loading activates β-catenin signaling in osteocytes.•Ovariectomy induced estrogen loss attenuates in vivo loading induced β-catenin signaling in osteocytes.•Changes in bone material and architectural properties do not appear to explain attenuated pathway activation.•Our data suggests estrogen loss alters the intrinsic ability of the osteocyte to respond to mechanical load.