A high-throughput liquid bead array assay confirms strong correlation between SARS-CoV-2 antibody level and COVID-19 severity

A detailed understanding of the adaptive host response to SARS-CoV-2 infection in humans is urgently needed. We developed a sensitive, high-throughput, and efficient assay using liquid bead array technology. We observed advantages over traditional ELISA for the detection and quantification of bindin...

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Bibliographic Details
Published iniScience Vol. 24; no. 2; p. 102052
Main Authors Bennett, Monique, Yoder, Sandra, Brady, Eric, Pulley, Jill M., Rhoads, Jillian P., Stewart, Thomas G., Bernard, Gordon R., Creech, C. Buddy, Wheeler, Allison P., Thomsen, Isaac
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 19.02.2021
Elsevier
Subjects
Biological Sciences
Microbiology
Viral Microbiology
Virology
Biological Sciences
Viral Microbiology
Microbiology
Virology
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Summary:A detailed understanding of the adaptive host response to SARS-CoV-2 infection in humans is urgently needed. We developed a sensitive, high-throughput, and efficient assay using liquid bead array technology. We observed advantages over traditional ELISA for the detection and quantification of binding IgG against the receptor binding domain (RBD) of SARS-CoV-2. To determine whether COVID-19 symptom severity correlates with SARS-CoV-2 IgG, we measured anti-RBD IgG levels from 67 subjects recovered from PCR-confirmed COVID-19. We found that COVID-19 symptom severity strongly correlated with RBD IgG level (p < 0.001). These findings have substantial implications for public policy surrounding assessments of antibody responses and possible immunity, as not all cases of COVID-19 can be assumed to generate a protective antibody response, and mild disease in particular is capable of generating very low-level anti-RBD IgG levels. These findings also have important implications for the selection of donors for convalescent plasma to be used therapeutically. [Display omitted] •We developed a sensitive, high-throughput assay for quantification of SARS-CoV-2 IgG•The assay uses liquid bead array technology for efficient and reproducible results•COVID-19 symptom severity was strongly correlated with SARS-CoV-2 SRBD IgG levels Biological Sciences; Microbiology; Virology; Viral Microbiology
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These authors contributed equally
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ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2021.102052