Physical restraint mouse models to assess immune responses under stress with or without habituation
Physical confinement, or restraint, is a psychological stressor used in rodent studies. A single restraint episode elevates blood corticosterone levels, a hallmark of stress responses. Repeated restraint results in habituation (or desensitization), whereas chronic exposure to unpredictable stressors...
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Published in | STAR protocols Vol. 2; no. 4; p. 100838 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
17.12.2021
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Physical confinement, or restraint, is a psychological stressor used in rodent studies. A single restraint episode elevates blood corticosterone levels, a hallmark of stress responses. Repeated restraint results in habituation (or desensitization), whereas chronic exposure to unpredictable stressors fails to induce habituation. Here, we provide our protocols and guidelines in using three mouse restraint models, namely prolonged restraint stress, repeated restraint stress, and chronic variable stress, to examine immunological homeostasis/competence, or lack thereof, under stress with or without habituation.
For complete information on the generation and use of these protocols, please refer to Rudak et al. (2021).
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•Three physical restraint mouse models to study the impact of long-term stress on immunity•A model of prolonged restraint stress altering immune homeostasis/competence•A model of repeated daily restraint stress resulting in habituation in animals•An optimized protocol for chronic variable stress circumventing habituation
Physical confinement, or restraint, is a psychological stressor used in rodent studies. A single restraint episode elevates blood corticosterone levels, a hallmark of stress responses. Repeated restraint results in habituation (or desensitization), whereas chronic exposure to unpredictable stressors fails to induce habituation. Here, we provide our protocols and guidelines in using three mouse restraint models, namely prolonged restraint stress, repeated restraint stress, and chronic variable stress, to examine immunological homeostasis/competence, or lack thereof, under stress with or without habituation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Technical contact Lead contact |
ISSN: | 2666-1667 2666-1667 |
DOI: | 10.1016/j.xpro.2021.100838 |