Repeating Structure of Cytoplasmic Poly(A)-Ribonucleoprotein

A repeating structure of cytoplasmic poly(A)-ribonucleoprotein is revealed by digestion with T2 RNase. A pattern of fragments that are multiples of about 27 residues is obtained. The repeating structure is readily reconstituted from purified poly(A) and cytoplasmic factors. Reconstitution is specifi...

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Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 77; no. 4; pp. 1890 - 1892
Main Authors Baer, Bradford W., Kornberg, Roger D.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.04.1980
National Acad Sciences
Subjects
Animals
Base Sequence
Binding Sites
Biochemistry
Cell lines
Cells, Cultured
Cytoplasm
Cytoplasm - metabolism
Electrophoresis
Gels
Mice
Molecular Weight
Nucleic acids
Nucleoproteins - metabolism
Poly A - metabolism
Ribonucleases - metabolism
Ribonucleoproteins
Ribonucleoproteins - metabolism
RNA
T-Phages - enzymology
Transfer RNA
Yeasts
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Summary:A repeating structure of cytoplasmic poly(A)-ribonucleoprotein is revealed by digestion with T2 RNase. A pattern of fragments that are multiples of about 27 residues is obtained. The repeating structure is readily reconstituted from purified poly(A) and cytoplasmic factors. Reconstitution is specific for poly(A), as shown by the lack of competition by poly(G), poly(C), poly(dA), and tRNA. The repeating structure is absent from the nucleus, and so appears to be formed upon transport to the cytoplasm.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.4.1890