In vitro and in vivo models for monkeypox

• Published in: iScience Vol. 26; no. 1; p. 105702
• Main Authors: Rosa, Rafael Borges, Ferreira de Castro, Emilene, Vieira da Silva, Murilo, Paiva Ferreira, Denise Caroline, Jardim, Ana Carolina Gomes, Santos, Igor Andrade, Marinho, Mikaela dos Santos, Ferreira França, Flávia Batista, Pena, Lindomar José
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.01.2023; Elsevier
• Subjects: Review; Virology; Virology
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2022.105702

The emergence and rapid spread outside of monkeypox virus (MPXV) to non-endemic areas has led to another global health emergency in the midst of the COVID-19 pandemic. The scientific community has sought to rapidly develop in vitro and in vivo models that could be applied in research with MPXV. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures. In vitro models are considered cost-effective and can be done in highly controlled conditions; however, they do not always resemble physiological conditions. In this way, several in vivo models are being characterized to meet the growing demand for new studies related to MPXV. In this review, we summarize the main MPXV models that have already been developed and discuss how they can contribute to advance the understanding of its pathogenesis, replication, and transmission, as well as identifying antivirals to treat infected patients. [Display omitted] Virology