Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy-L-Sorbose

• Published in: Applied and Environmental Microbiology Vol. 74; no. 16; pp. 5250 - 5253
• Main Authors: Yang, Xue-Peng, Wei, Liu-Jing, Lin, Jin-Ping, Yin, Bo, Wei, Dong-Zhi
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2008; American Society for Microbiology (ASM)
• Subjects: Amino Acid Sequence; Bacteria; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Bacteriology; Biochemistry; Biological and medical sciences; Complementation; Fundamental and applied biological sciences. Psychology; Genes; Genetic Complementation Test; Genetic Vectors; Gluconobacter oxydans; Gluconobacter oxydans - enzymology; Gluconobacter oxydans - genetics; L-Iditol 2-Dehydrogenase - isolation & purification; L-Iditol 2-Dehydrogenase - metabolism; Membrane Proteins - metabolism; Membranes; Microbiology; Oxidation-Reduction; Physiology and Biotechnology; Plasmids; PQQ Cofactor - metabolism; Proteins; Sorbitol - metabolism; Sorbose - analogs & derivatives; Sorbose - biosynthesis; Acetobacteraceae; Gluconobacter oxydans; Enzyme; Quinone; Production; Bacteria; Membrane; Oxidoreductases; Dehydrogenase
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.00272-08

A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.