circadian oscillator gene GIGANTEA mediates a long-term response of the Arabidopsis thaliana circadian clock to sucrose

Circadian clocks are 24-h timing devices that phase cellular responses; coordinate growth, physiology, and metabolism; and anticipate the day-night cycle. Here we report sensitivity of the Arabidopsis thaliana circadian oscillator to sucrose, providing evidence that plant metabolism can regulate cir...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 108; no. 12; pp. 5104 - 5109
Main Authors Dalchau, Neil, Baek, Seong J, Briggs, Helen M, Robertson, Fiona C, Dodd, Antony N, Gardner, Michael J, Stancombe, Matthew A, Haydon, Michael J, Stan, Guy-Bart, Gonçalves, Jorge M, Webb, Alex A.R
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 22.03.2011
National Acad Sciences
Subjects
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Biological Sciences
Cells
Circadian Clocks - drug effects
Circadian Clocks - physiology
Circadian rhythm
Circadian Rhythm - drug effects
Circadian Rhythm - physiology
Circadian rhythms
Darkness
Data processing
Feedback
Flowers & plants
Gene expression
genes
Luminescence
Mathematical models
Messenger RNA
Metabolism
Modeling
Models, Biological
Oscillators
Parametric models
Plant growth regulators
Plants
prediction
Seedlings
Sucrose
Sucrose - metabolism
Sucrose - pharmacology
Online AccessGet full text

Cover

More Information
Summary:Circadian clocks are 24-h timing devices that phase cellular responses; coordinate growth, physiology, and metabolism; and anticipate the day-night cycle. Here we report sensitivity of the Arabidopsis thaliana circadian oscillator to sucrose, providing evidence that plant metabolism can regulate circadian function. We found that the Arabidopsis circadian system is particularly sensitive to sucrose in the dark. These data suggest that there is a feedback between the molecular components that comprise the circadian oscillator and plant metabolism, with the circadian clock both regulating and being regulated by metabolism. We used also simulations within a three-loop mathematical model of the Arabidopsis circadian oscillator to identify components of the circadian clock sensitive to sucrose. The mathematical studies identified GIGANTEA (GI) as being associated with sucrose sensing. Experimental validation of this prediction demonstrated that GI is required for the full response of the circadian clock to sucrose. We demonstrate that GI acts as part of the sucrose-signaling network and propose this role permits metabolic input into circadian timing in ARABIDOPSIS:
Bibliography:http://dx.doi.org/10.1073/pnas.1015452108
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
Author contributions: N.D., G.-B.S., J.M.G., and A.A.W. designed research; N.D., S.J.B., H.M.B., F.C.R., A.N.D., M.J.G., M.A.S., and M.J.H. performed research; N.D., S.J.B., H.M.B., and F.C.R. analyzed data; and N.D., G.-B.S., J.M.G., and A.A.R.W. wrote the paper.
2Present address: Plant Functional Genomics Research Group, Plant Genomic Network Research Team, RIKEN, Tsurumi-ku, Yokohama City, Kanagawa 230-0045, Japan.
Edited by Steve A. Kay, University of California at San Diego, La Jolla, CA, and approved January 26, 2011 (received for review October 16, 2010).
3Present address: Department of Biology, University of York, York YO10 5DD, United Kingdom.
4Present address: European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, United Kingdom.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1015452108