Protective effect of carboxymethyl-glucan (CM-G) against DNA damage in patients with advanced prostate cancer

• Published in: Genetics and molecular biology Vol. 34; no. 1; pp. 131 - 135
• Main Authors: Magnani, Marciane, Castro-Gomez, Raul Jorge Hernan, Mori, Mateus Prates, Kuasne, Hellen, Gregório, Emerson Pereira, Libos, Jr, Farid, de Syllos Cólus, Ilce Mara
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Sociedade Brasileira de Genética 01.01.2011
• Subjects: antioxidant; BIOCHEMISTRY & MOLECULAR BIOLOGY; carboxymethyl-glucan; GENETICS & HEREDITY; Mutagenesis; prostate cancer; β-D-glucan; antioxidant; prostate cancer; β-D-glucan; carboxymethyl-glucan
• ISSN: 1415-4757; 1678-4685; 1678-4685
• DOI: 10.1590/S1415-47572010005000103

Carboxymethyl-glucan (CM-G) is a soluble derivative from Saccharomyces cerevisiae (1 → 3)(1 → 6)-β-D-glucan. The protective efficiency of CM-G against DNA damage in cells from patients with advanced prostate cancer (PCa), and undergoing Androgen Deprivation Therapy (ADT), was evaluated. DNA damage scores were obtained by the comet assay, both before and after treatment with CM-G. The reduction in DNA damage, ranging from 18% to 87%, with an average of 59%, was not related to the increased number of leukocytes in peripheral blood. The results demonstrate for the first time the protective effect of CM-G against DNA damage in patients with advanced PCa. Among smokers, three presented the highest reduction in DNA damage after treatment with CM-G. There was no observable relationship between DNA damage scores before and after treatment, and age, alcoholism and radiotherapy.