Anti-angiotensin converting enzyme (ACE) proteins from mycelia of Ganoderma lucidum (Curtis) P. Karst

• Published in: BMC complementary and alternative medicine Vol. 13; no. 1; p. 256
• Main Authors: Mohamad Ansor, Nurhuda, Abdullah, Noorlidah, Aminudin, Norhaniza
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.10.2013; BioMed Central
• Subjects: ACE inhibitors; Acids; Analysis of Variance; Angiotensin converting enzyme; Angiotensin-Converting Enzyme Inhibitors - chemistry; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Blood pressure; Cardiovascular disease; Care and treatment; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Enzymes; Fermentation; Fungal Proteins - chemistry; Fungal Proteins - metabolism; Health aspects; High performance liquid chromatography; Hypertension; Laboratories; Medicinal plants; Mycelia; Mycelium - chemistry; Mycelium - enzymology; Peptides; Peptidyl-Dipeptidase A - chemistry; Peptidyl-Dipeptidase A - metabolism; Physiological aspects; Precipitation (Meteorology); Proteins; Proteomics; Reishi; Reishi - chemistry; Reishi - enzymology; Rodents; Smooth muscle; Studies
• ISSN: 1472-6882; 1472-6882
• DOI: 10.1186/1472-6882-13-256

Ganoderma lucidum has been purported as a potent remedy in the treatment and prevention of several ailments, including hypertension. This study aimed to explore the anti-ACE potential of protein fractions from the mycelia of G. lucidum. Ganoderma lucidum mycelia were cultivated by submerged fermentation in a liquid medium containing brown sugar and spent brewer's yeast. Intracellular proteins were fractionated from mycelia crude water extract by ammonium sulphate precipitation, and their angiotensin converting enzyme inhibitory activity was evaluated. The potential anti-ACE protein fractions were further separated by RP-HPLC and characterised using proteomics platforms. Preliminary result demonstrated that the mycelia crude water extract inhibited ACE at IC₅₀ value of 1.134 ± 0.036 mg/mL. Following protein fractionation and HPLC purification, the presence of highly potential anti-ACE proteins with the IC₅₀ values less than 200 μg/mL was detected. Characterisation of these proteins demonstrated the presence of four different antihypertensive-related proteins involved in the regulation of blood pressure through different mechanisms. This study suggests that the mycelia of G. lucidum has high potential in lowering blood pressure level due to the presence of several antihypertensive-related proteins such as cystathionine beta synthase-like protein, DEAD/DEAH box helicase-like protein, paxillin-like protein, and alpha/beta hydrolase-like protein.