Transcriptional Regulation of the Yeast Cytochrome c Gene

DNA from the cloned yeast iso-1-cytochrome c, cyc1, gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcri...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 76; no. 8; pp. 3627 - 3631
Main Authors Zitomer, Richard S., Montgomery, Donna L., Nichols, Diane L., Hall, Benjamin D.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.08.1979
National Acad Sciences
Subjects
Cell growth
Cytochrome c Group - genetics
Cytochromes
DNA
DNA, Recombinant
Genes
Glucose - metabolism
Half lives
Messenger RNA
Nucleic Acid Hybridization
Plasmids
Raffinose - metabolism
Repression
RNA
RNA, Messenger - metabolism
Saccharomyces cerevisiae - genetics
Transcription, Genetic
Yeasts
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Summary:DNA from the cloned yeast iso-1-cytochrome c, cyc1, gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cyc1 gene was repressed within 2.5 min. The half-life of hybridizable cyc1 RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cyc1 gene is subject to transcriptional regulation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.8.3627