Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes

• Published in: eLife Vol. 12
• Main Authors: Andolina, Chiara, Graumans, Wouter, Guelbeogo, Moussa, van Gemert, Geert-Jan, Ramijth, Jordache, Harouna, Soré, Soumanaba, Zongo, Stoter, Rianne, Vegte-Bolmer, Marga, Pangos, Martina, Sinnis, Photini, Collins, Katharine, Staedke, Sarah G, Tiono, Alfred B, Drakeley, Chris, Lanke, Kjerstin, Bousema, Teun
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 22.03.2024; eLife Sciences Publications Ltd
• Subjects: Anopheles; Anopheles stephensi; Culicidae; Exocrine glands; expelling; Experiments; gametocyte; Gametocytes; Infections; Malaria; Medical research; Medicine, Experimental; Mosquitoes; Oocysts; Parasites; Plasmodium falciparum; Protozoa; Salivary gland; Skin; Sporogony; sporozoite; Sporozoites; Netherlands; Pakistan; Burkina Faso; anopheles; infectious disease; P. falciparum; microbiology; sporogony; gametocyte; sporozoite; expelling
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.90989

It is currently unknown whether all -infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34-501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171-2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential.