Serum and knee synovial fluid matrixmetalloproteinase-13 and tumor necrosis factor-alpha levels in patients with late stage osteoarthritis

• Published in: Acta orthopaedica et traumatologica turcica Vol. 50; no. 6; pp. 670 - 673
• Main Authors: Özler, Kenan, Aktaş, Erdem, Atay, Çiğdem, Yılmaz, Barış, Arıkan, Murat, Güngör, Şafak
• Format: Journal Article
• Language: English
• Published: Turkey Elsevier B.V 01.12.2016; Turkish Association of Orthopaedics and Traumatology; AVES
• Subjects: Aged; Arthroplasty, Replacement, Knee - methods; Female; Humans; Knee osteoarthritis; Male; Matrix metalloproteinase; Matrix Metalloproteinase 13 - blood; Matrix Metalloproteinase 13 - chemistry; Middle Aged; Original; Osteoarthritis, Knee - surgery; Perioperative Period; Serologic Tests; Synovial Fluid - chemistry; TNF-alpha; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - chemistry; TNF-alpha; Knee osteoarthritis; Matrix metalloproteinase
• ISSN: 1017-995X; 2589-1294
• DOI: 10.1016/j.aott.2015.11.003

To compare the levels of MMP-13 and TNF-α in late stage osteoarthritis, define their predominant pathways and investigate their correlation with McMaster Universities Arthritis Index scores. A total of 42 patients (mean age 64 ± 8.8) with grade 3 and grade 4 knee osteoarthritis according to Kellegren- Lawrence criteria and who were scheduled for total knee arthroplasty were enrolled in the study. TNF-alpha and MMP-13 levels were measured preoperatively from venous blood samples and intraoperatively from knee synovial fluid via ELISA. Preoperative and 1 month postoperative knee functions were assessed by McMaster Universities Arthritis Index. Grade 4 synovial fluid MMP-13 (4.76 ± 5.82) was elevated compared to grade 3 (3.95 ± 4.45) (p = 0.438), whereas grade 3 serum MMP-13 (1.128 ± 0.308) was found elevated compared to grade 4 (1.038 ± 0.204) (p = 0.430). Grade 4 serum TNF-α (0.253 ± 0.277) was elevated compared to grade 3 (0.206 ± 0.219) whereas grade 3 synovial fluid TNF-α (0.129 ± 0.052) was elevated compared to grade 4 (0.118 ± 0.014). Positive correlation was observed between synovial fluid MMP-13 levels and postoperative WOMAC scores. Mean serum TNF-α level (0.226 ± 0.246 pg/ml) was found higher compared to synovial level (0.124 ± 1.59), synovial MMP-13 level (4.31 ± 1.24) was found higher compared to serum level (1.089 ± 1.519). Despite the systemic increase in TNF-α levels concordant with osteoarthritis grade, MMP-13 levels are elevated via local manner with a significant correlation with WOMAC scores. Level IV, Diagnostic study.