Somatic Evolution in Non-neoplastic IBD-Affected Colon

• Published in: Cell Vol. 182; no. 3; pp. 672 - 684.e11
• Main Authors: Olafsson, Sigurgeir, McIntyre, Rebecca E., Coorens, Tim, Butler, Timothy, Jung, Hyunchul, Robinson, Philip S., Lee-Six, Henry, Sanders, Mathijs A., Arestang, Kenneth, Dawson, Claire, Tripathi, Monika, Strongili, Konstantina, Hooks, Yvette, Stratton, Michael R., Parkes, Miles, Martincorena, Inigo, Raine, Tim, Campbell, Peter J., Anderson, Carl A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.08.2020; Cell Press
• Subjects: Adult; Aged; Aged, 80 and over; Aging - genetics; Clonal Evolution - genetics; Clonal Evolution - immunology; Colitis - genetics; Colitis - metabolism; Colitis, Ulcerative - genetics; Colitis, Ulcerative - metabolism; Crohn Disease - genetics; Crohn Disease - metabolism; Crohn's disease; DNA-Binding Proteins - genetics; Epithelial Cells - metabolism; Epithelial Cells - pathology; F-Box-WD Repeat-Containing Protein 7 - genetics; Female; Humans; IL17; INDEL Mutation; inflammatory bowel disease; Inflammatory Bowel Diseases - genetics; Inflammatory Bowel Diseases - immunology; Inflammatory Bowel Diseases - metabolism; Inflammatory Bowel Diseases - pathology; Interleukin-17 - metabolism; intestinal epithelia; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Male; Middle Aged; Mutation Rate; mutational signatures; Phylogeny; PIGR; Point Mutation; Receptors, Cell Surface - genetics; Ribonucleases - genetics; somatic mutations; Toll-Like Receptors - genetics; Transcription Factors - genetics; ulcerative colitis; Whole Genome Sequencing; ZC3H12A; somatic mutations; PIGR; mutational signatures; ZC3H12A; mutation rate; Crohn's disease; ulcerative colitis; intestinal epithelia; IL17; inflammatory bowel disease
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/j.cell.2020.06.036

Inflammatory bowel disease (IBD) is a chronic inflammatory disease associated with increased risk of gastrointestinal cancers. We whole-genome sequenced 446 colonic crypts from 46 IBD patients and compared these to 412 crypts from 41 non-IBD controls from our previous publication on the mutation landscape of the normal colon. The average mutation rate of affected colonic epithelial cells is 2.4-fold that of healthy colon, and this increase is mostly driven by acceleration of mutational processes ubiquitously observed in normal colon. In contrast to the normal colon, where clonal expansions outside the confines of the crypt are rare, we observed widespread millimeter-scale clonal expansions. We discovered non-synonymous mutations in ARID1A, FBXW7, PIGR, ZC3H12A, and genes in the interleukin 17 and Toll-like receptor pathways, under positive selection in IBD. These results suggest distinct selection mechanisms in the colitis-affected colon and that somatic mutations potentially play a causal role in IBD pathogenesis. [Display omitted] •IBD-affected colons accrue substitutions and indels 2.4 and 7 times faster than normal•17 signatures of mutational processes, including treatment•Millimeter-scale clonal expansions late in molecular time•Distinct mechanisms of positive selection of mutations in immune-related genes Whole-genome sequencing of inflammatory bowel disease patient samples allows insight into mutational burdens and processes associated with disease, including putative driver mutations positively selected in the diseased colon.