Salivary nitric oxide, Simplified Oral Hygiene Index, and salivary flow rate in smokers and non-smokers: a cross-sectional study [version 2; peer review: 2 approved]

• Published in: F1000 research Vol. 8; p. 1744
• Main Authors: Bachtiar, Endang Winiati, Putri, Atikah Cyntia, Bachtiar, Boy Muchlis
• Format: Journal Article
• Language: English
• Published: London Faculty of 1000 Ltd 01.01.2019; F1000 Research Limited; F1000 Research Ltd
• Subjects: Cigarettes; Cross-sectional studies; Dentistry; Enzymes; Ethics; Light; Nicotine; Nitric oxide; Oral cavity; Oral hygiene; Physiology; Saliva; Smoking; Systemic diseases; United Kingdom > UK; Indonesia; Salivary Flow Rate; Griess Method; Salivary Nitric Oxide; Smoker; Whole Saliva; Oral Hygiene Index
• ISSN: 2046-1402; 2046-1402
• DOI: 10.12688/f1000research.20099.2

Background: Salivary nitric oxide plays an important role as an antibacterial agent in the oral cavity. Here, we analyze salivary nitric oxide, Simplified Oral Hygiene Index (OHI-S) scores and the salivary flow rate in smokers and non-smokers which has not been done previously. Methods: A cross sectional study included 25 smokers and 25 non-smokers. Their OHI-S results were categorized as "good," "medium," or "bad." Unstimulated saliva samples were collected, and their nitric oxide concentration was measured using the Griess method. Results: The salivary flow rate in smokers was lower, at 0.30 ml/minute, compared to non-smokers who had a salivary flow rate of 0.33 ml/minute. This was statistically insignificant. There was a significant difference in the concentrations of nitric oxide between smokers and non-smokers (p < 0.05). Smokers had higher concentrations than non-smokers (185.4 µM Vs 114.60 µM). In addition, there was a moderate positive correlation (r = 0.305) between the concentration of salivary nitric oxide level and the OHI-S results. Conclusions: It was concluded that salivary nitric oxide concentration was higher in smokers, and the oral hygiene condition of smokers was poor.