Possible stochastic sex determination in Bursaphelenchus nematodes

• Published in: Nature communications Vol. 13; no. 1; p. 2574
• Main Authors: Shinya, Ryoji, Sun, Simo, Dayi, Mehmet, Tsai, Isheng Jason, Miyama, Atsushi, Chen, Anthony Fu, Hasegawa, Koichi, Antoshechkin, Igor, Kikuchi, Taisei, Sternberg, Paul W.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.05.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 38/91; 45; 45/23; 45/70; 631/181/2474; 631/208/135; 631/208/212/748; 64; Animals; Bursaphelenchus; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Chromosomes; Compensation; Deposition; Differentiation; Disorders of Sex Development - genetics; Dosage; Dosage compensation; Dosage Compensation, Genetic; Environmental factors; Epigenesis, Genetic; Epigenetics; Female; Gene sequencing; Genetic analysis; Genetic screening; Genomes; Humanities and Social Sciences; Humans; Male; multidisciplinary; Nematoda - genetics; Nematoda - metabolism; Nematodes; Phenotypes; Science; Science (multidisciplinary); Sex; Sex determination; Sex differentiation; Stochasticity; X Chromosome - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-30173-2

Sex determination mechanisms evolve surprisingly rapidly, yet little is known in the large nematode phylum other than for Caenorhabditis elegans , which relies on chromosomal XX-XO sex determination and a dosage compensation mechanism. Here we analyze by sex-specific genome sequencing and genetic analysis sex determination in two fungal feeding/plant-parasitic Bursaphelenchus nematodes and find that their sex differentiation is more likely triggered by random, epigenetic regulation than by more well-known mechanisms of chromosomal or environmental sex determination. There is no detectable difference in male and female chromosomes, nor any linkage to sexual phenotype. Moreover, the protein sets of these nematodes lack genes involved in X chromosome dosage counting or compensation. By contrast, our genetic screen for sex differentiation mutants identifies a Bursaphelenchus ortholog of tra-1 , the major output of the C. elegans sex determination cascade. Nematode sex determination pathways might have evolved by “bottom-up” accretion from the most downstream regulator, tra-1 . In most species, sex is determined by genetic or environmental factors. Here, the authors present evidence that sex determination in Bursaphelenchus nematodes is instead likely to be regulated by a random, epigenetic mechanism.