Identification of Campylobacter jejuni Genes Involved in the Response to Acidic pH and Stomach Transit

• Published in: Applied and Environmental Microbiology Vol. 74; no. 5; pp. 1583 - 1597
• Main Authors: Reid, Anne N, Pandey, Reenu, Palyada, Kiran, Naikare, Hemant, Stintzi, Alain
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.2008; American Society for Microbiology (ASM)
• Subjects: acid tolerance; acidity; Alkanesulfonates; Animals; Bacteria; Bacteriology; Biological and medical sciences; Campylobacter jejuni; Campylobacter jejuni - genetics; carbohydrate metabolism; DNA Primers - genetics; electrons; energy; Food Microbiology; Fundamental and applied biological sciences. Psychology; gastroenteritis; Gastrointestinal diseases; Gastrointestinal Transit - genetics; gene expression; gene expression regulation; Gene Expression Regulation, Bacterial; Genes; Genes, Bacterial - genetics; Genetics; heat stress; hosts; Hydrogen-Ion Concentration; Microbiology; Microscopy, Electron, Scanning; molecular chaperones; nitrites; Oligonucleotide Array Sequence Analysis; oxidative stress; piglets; polysaccharides; Reverse Transcriptase Polymerase Chain Reaction; ribosomal proteins; Stomach; stress response; Sus scrofa; transcription (genetics); Stomach; Gene; Campylobacteraceae; pH; Bacteria; Identification; Campylobacter jejuni
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01507-07

Campylobacter jejuni causes food- and waterborne gastroenteritis, and as such it must survive passage through the stomach in order to reach the gastrointestinal tract. While little is known about how C. jejuni survives transit through the stomach, its low infectious dose suggests it is well equipped to sense and respond to acid shock. In this study, the transcriptional profile of C. jejuni NCTC 11168 was obtained after the organism was exposed to in vitro and in vivo (piglet stomach) acid shock. The observed down-regulation of genes encoding ribosomal proteins likely reflects the need to reshuffle energy toward the expression of components required for survival. Acid shock also caused C. jejuni to up-regulate genes involved in stress responses. These included heat shock genes as well as genes involved in the response to oxidative and nitrosative stress. A role for the chaperone clpB in acid resistance was confirmed in vitro. Some genes showed expression patterns that were markedly different in vivo and in vitro, which likely reflects the complexity of the in vivo environment. For instance, transit through the stomach was characterized by up-regulation of genes that encode products that are involved in the use of nitrite as a terminal electron acceptor and down-regulation of genes that are involved in capsular polysaccharide expression. In conclusion, this study has enabled us to understand how C. jejuni modulates gene expression in response to acid shock in vitro and to correlate this with gene expression profiles of C. jejuni as it transits through the host stomach.