DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity

• Published in: International journal of molecular sciences Vol. 21; no. 11; p. 3748
• Main Authors: Treffel, Loïc, Navasiolava, Nastassia, Mkhitaryan, Karen, Jouan, Emmanuelle, Zuj, Kathryn, Gauquelin-Koch, Guillemette, Custaud, Marc-Antoine, Gharib, Claude
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 26.05.2020; MDPI AG
• Subjects: ADC diffusion; Adult; Back Pain - etiology; Back Pain - prevention & control; Body Water - metabolism; Compression Bandages; Humans; Intervertebral Disc - diagnostic imaging; Intervertebral Disc - metabolism; intradiscal proteins; Lumbosacral Region - diagnostic imaging; Male; Proteoglycans - metabolism; space physiology; spine; T1-T2 mapping; Thigh - blood supply; Vasoconstriction; vertebral deconditioning; Weightlessness - adverse effects; vertebral deconditioning; ADC diffusion; back pain; T1-T2 mapping; spine; intradiscal proteins; space physiology
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21113748

Most astronauts experience back pain after spaceflight, primarily located in the lumbar region. Intervertebral disc herniations have been observed after real and simulated microgravity. Spinal deconditioning after exposure to microgravity has been described, but the underlying mechanisms are not well understood. The dry immersion (DI) model of microgravity was used with eighteen male volunteers. Half of the participants wore thigh cuffs as a potential countermeasure. The spinal changes and intervertebral disc (IVD) content changes were investigated using magnetic resonance imaging (MRI) analyses with T1-T2 mapping sequences. IVD water content was estimated by the apparent diffusion coefficient (ADC), with proteoglycan content measured using MRI T1-mapping sequences centered in the nucleus pulposus. The use of thigh cuffs had no effect on any of the spinal variables measured. There was significant spinal lengthening for all of the subjects. The ADC and IVD proteoglycan content both increased significantly with DI (7.34 ± 2.23% and 10.09 ± 1.39%, respectively; mean ± standard deviation), < 0.05). The ADC changes suggest dynamic and rapid water diffusion inside IVDs, linked to gravitational unloading. Further investigation is needed to determine whether similar changes occur in the cervical IVDs. A better understanding of the mechanisms involved in spinal deconditioning with spaceflight would assist in the development of alternative countermeasures to prevent IVD herniation.