2-Propanol in the mobile phase reduces the time of analysis of CLA isomers by silver ion-HPLC

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 878; no. 1; pp. 88 - 91
• Main Authors: Kuhnt, Katrin, Degen, Christian, Jahreis, Gerhard
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.2010; Elsevier
• Subjects: 2-Propanol - chemistry; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - economics; Chromatography, High Pressure Liquid - methods; Conjugated linoleic acids; Fundamental and applied biological sciences. Psychology; General pharmacology; Geometric isomers; Isomerism; Linoleic Acids, Conjugated - analysis; Medical sciences; Pharmacology. Drug treatments; Sensitivity and Specificity; Silver - chemistry; Silver ion-high performance liquid chromatography; Statistics, Nonparametric; Time Factors; Time of analysis; Time of analysis; Conjugated linoleic acids; Silver ion-high performance liquid chromatography; Geometric isomers; Conjugated linoleic acid; Stereoisomer; Polyunsaturated fatty acid; HPLC chromatography; Lipids; Mobile phase; Isomer; chromatography; Conjugated dienic compound; Silver ion; Silver ion-high performance liquid; Execution time
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2009.11.006

Individual isomers of octadecadienoic acid (C18:2) with conjugated double bonds (conjugated linoleic acids; CLA) exert different biological activities. Their distribution in food and tissues differs. Therefore, the separation of the various positional and geometric isomers is important. The time of analysis using silver ion-high performance liquid chromatography can extend up to 90 min. The aim of this study was to reduce this time. The time of analysis reduced from ca. 90 min onto 45 to 35 min, respectively, by the addition of 0.05% or 0.1% (v/v) 2-propanol to the mobile phase [acetonitrile (0.1%; v/v) and diethyl ether (0.5%; v/v) in n-hexane]. There was no effect on resolution of the 17 individual CLA isomers of the CLA mixture. Regarding the lowest coefficient of variation and an adequate baseline separation the use of 0.05% 2-propanol in the mobile phase is recommended, without any disadvantages and adverse effects on the service life of columns. In conclusion, adding 0.05% or 0.1% 2-propanol to the mobile phase shortens the time of analysis of CLA isomers, saves solvents and reduces costs.