Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet

• Published in: Applied microbiology and biotechnology Vol. 84; no. 2; pp. 341 - 347
• Main Authors: Wang, Yanping, Xu, Nv, Xi, Aodeng, Ahmed, Zaheer, Zhang, Bin, Bai, Xiaojia
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.08.2009; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Animals; Applied Microbial and Cell Physiology; Bacteria; Bile; Biological and medical sciences; Biotechnology; Body Weight; Cholesterol; Cholesterol - administration & dosage; Cultured Milk Products - microbiology; Diet; E coli; Eating; Effects; Escherichia coli; Feces; Feces - chemistry; Feces - microbiology; Fundamental and applied biological sciences. Psychology; High density lipoprotein; Intestines - metabolism; Intestines - microbiology; Laboratory animals; Lactobacillus plantarum; Lactobacillus plantarum - isolation & purification; Lactobacillus plantarum - metabolism; Life Sciences; Lipid Metabolism; Lipids; Lipids - blood; Lipoproteins; Liver; Male; Metabolism; Microbial Genetics and Genomics; Microbiology; Milk; Organic acids; Physiology; Probiotics; Rats; Rats, Sprague-Dawley; Ribosomal DNA; Studies; Tibet; Triglycerides; Tibet; Cholesterol-lowering effect; Kefir; MA2; Lactic acid bacteria; Lactobacillus plantarum; Rat; Rodentia; Gut; Lipids; Microflora; Metabolism; Cholesterol; Vertebrata; Mammalia; Diet; Bacteria; Lactobacillaceae; Lactobacillus plantarum MA2
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-009-2012-x

The objective of this study was to evaluate the effects of Lactobacillus plantarum MA2, an isolate from Chinese traditional Tibet kefir, on cholesterol-lowering and microflora of rat in vivo. Rats were fed on cholesterol-enriched experimental diet, supplemented with lyophilized L. plantarum MA2 powder, with a dose of 10¹¹ cells/day per mice. The results showed that L. plantarum MA2 feeding significantly lowered serum total cholesterol, low-density lipoprotein cholesterol, and triglycerides level, while there was no change in high-density lipoprotein cholesterol. In addition, liver total cholesterol and triglycerides was also decreased. However, fecal cholesterol and triglycerides was increased significantly (P < 0.05) in comparison with the control. Also, L. plantarum MA2 increased the population of lactic acid bacteria and bifidobacteria in the fecal, but it did not change the number of Escherichia coli as compared to control. Moreover, pH, moisture, and organic acids in the fecal were also measured. The present results indicate the probiotic potential of the L. plantarum MA2 strain in hypocholesterolemic effect and also increasing the probiotic count in the intestine.