Principles of Cotranslational Ubiquitination and Quality Control at the Ribosome

• Published in: Molecular cell Vol. 50; no. 3; pp. 379 - 393
• Main Authors: Duttler, Stefanie, Pechmann, Sebastian, Frydman, Judith
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.05.2013
• Subjects: eukaryotic cells; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; Peptides - genetics; Peptides - metabolism; polypeptides; Protein Biosynthesis; Protein Folding; Quality Control; ribosomes; Ribosomes - genetics; Ribosomes - metabolism; RNA, Messenger - genetics; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; ubiquitin-protein ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Ubiquitination
• ISSN: 1097-2765; 1097-4164; 1097-4164
• DOI: 10.1016/j.molcel.2013.03.010

Achieving efficient cotranslational folding of complex proteomes poses a challenge for eukaryotic cells. Nascent polypeptides that emerge vectorially from the ribosome often cannot fold stably and may be susceptible to misfolding and degradation. The extent to which nascent chains are subject to cotranslational quality control and degradation remains unclear. Here, we directly and quantitatively assess cotranslational ubiquitination and identify, at a systems level, the determinants and factors governing this process. Cotranslational ubiquitination occurs at very low levels and is carried out by a complex network of E3 ubiquitin ligases. Ribosome-associated chaperones and cotranslational folding protect the majority of nascent chains from premature quality control. Nonetheless, a number of nascent chains whose intrinsic properties hinder efficient cotranslational folding remain susceptible for cotranslational ubiquitination. We find that quality control at the ribosome is achieved through a tiered system wherein nascent polypeptides have a chance to fold before becoming accessible to ubiquitination. [Display omitted] ► We assess the determinants of cotranslational ubiquitination in vivo ► Ubiquitination is linked to nascent chain properties that challenge their folding ► Cotranslational folding and chaperones protect nascent chains from ubiquitination ► Ribosome-bound and cytosolic E3 ligases mediate cotranslational ubiquitination