High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)
Key message Two novel resistant QTLs mapped and candidate genes identified for Aspergillus flavus resistance in cultivated peanut using SLAF-seq. Aflatoxin contamination in peanuts caused by Aspergillus flavus is a serious food safety issue for human health around the world. Host plant resistance to...
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Published in | Theoretical and applied genetics Vol. 133; no. 7; pp. 2239 - 2257 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.07.2020
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
Two novel resistant QTLs mapped and candidate genes identified for
Aspergillus flavus
resistance in cultivated peanut using SLAF-seq.
Aflatoxin contamination in peanuts caused by
Aspergillus flavus
is a serious food safety issue for human health around the world. Host plant resistance to fungal infection and reduction in aflatoxin are crucial for mitigating this problem. Identification of the resistance-linked markers can be used in marker-assisted breeding for varietal development. Here we report construction of two high-density genetic linkage maps with 1975 SNP loci and 5022 SNP loci, respectively. Two consistent quantitative trait loci (QTL) were identified as
qRAF
-
3
-
1
and
qRAF
-
14
-
1
, which located on chromosomes A03 and B04, respectively. QTL
qRAF
-
3
-
1
was mapped within 1.67 cM and had more than 19% phenotypic variance explained (PVE), while
qRAF
-
14
-
1
was located within 1.34 cM with 5.15% PVE. While comparing with the reference genome, the mapped QTLs,
qRAF
-
3
-
1
and
qRAF
-
14
-
1
, were located within a physical distance of 1.44 Megabase pair (Mbp) and 2.22 Mbp, harboring 67 and 137 genes, respectively. Among the identified candidate genes, six genes with the same function were found within both QTLs regions. In addition, putative disease resistance RPP13-like protein 1 (RPP13), lipoxygenase (Lox), WRKY transcription factor (WRKY) and cytochrome P450 71B34 genes were also identified. Using microarray analysis, genes responded to
A
.
flavus
infection included coding for RPP13, pentatricopeptide repeat-containing-like protein, and Lox which may be possible candidate genes for resistance to
A
.
flavus
. The QTLs and candidate genes will further facilitate marker development and validation of genes for deployment in the molecular breeding programs against
A
.
flavus
in peanuts. |
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ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-020-03594-0 |