Peculiarities of Cell Seeding on Electroformed Polycaprolactone Scaffolds Modified with Surface-Active Agents Triton X-100 and Polyvinylpyrrolidone

We compared the capability of human fibroblasts to populate porous polycaprolactone (PCL) scaffolds modified during fabrication with surface-active agents Triton Х-100 (type 1 scaffold) and polyvinylpyrrolidone (type 2 scaffold). The mean fiber diameter in both scaffolds was almost the same: 3.90±2....

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Published inBulletin of experimental biology and medicine Vol. 169; no. 4; pp. 600 - 604
Main Authors Afanasiev, S. A., Muslimova, E. F., Nashchekina, Yu. A., Nikonov, P. O., Rogovskaya, Yu. V., Tenchurin, T. Kh, Nesterenko, E. V., Grakova, E. V., Kopeva, K. V., Akhmedov, S. D.
Format Journal Article
LanguageEnglish
Published New York Springer US 01.08.2020
Springer
Subjects
Biomedical and Life Sciences
Biomedicine
Cell Biology
Internal Medicine
Laboratory Medicine
Pathology
Povidone
Surface active agents
Tissue engineering
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
tissue engineering
synthetic scaffold
Triton Х-100
cell culture
polyvinylpyrrolidone
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Summary:We compared the capability of human fibroblasts to populate porous polycaprolactone (PCL) scaffolds modified during fabrication with surface-active agents Triton Х-100 (type 1 scaffold) and polyvinylpyrrolidone (type 2 scaffold). The mean fiber diameter in both scaffolds was almost the same: 3.90±2.19 and 2.46±2.15 μ, respectively. Type 1 scaffold had higher surface density and hydrophilicity, when type 2 scaffold was 1.6 times thicker. The cells were seeded on the scaffolds by the dynamic seeding technique and then cultured in Petri dishes with nutrient medium in a humid atmosphere. During 3-day culturing, no cell release from the matrix was noted. DAPI staining proved the presence of cells in both scaffolds. However, in type 1 scaffold the cells populated the whole thickness, while in type 2 scaffold, the cells were present only in the superficial layer. These findings suggest that PCL scaffolds modified with Triton Х-100 or polyvinylpyrrolidone are not cytotoxic, but the structure of the scaffold treated with Triton Х-100 is more favorable for population with cells.
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ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-020-04936-0