Adherence of Enterohemorrhagic Escherichia coli O157, O26, and O111 Strains to Bovine Intestinal Explants Ex Vivo

We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adher...

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Published inApplied and Environmental Microbiology Vol. 73; no. 9; pp. 3084 - 3090
Main Authors Girard, Francis, Dziva, Francis, van Diemen, Pauline, Phillips, Alan D, Stevens, Mark P, Frankel, Gad
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.05.2007
Subjects
Adhesins, Bacterial - metabolism
Animals
Bacterial Adhesion
Bacteriology
Biological and medical sciences
Cattle
Digestive system
E coli
Enterohemorrhagic Escherichia coli - physiology
Escherichia coli
Escherichia coli Proteins - metabolism
Food Microbiology
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Intestinal Mucosa - metabolism
Intestines - microbiology
Microbiology
Receptors, Cell Surface - metabolism
Species Specificity
Tissue Culture Techniques
Vertebrata
Mammalia
Bovine
Ex vivo
Escherichia coli
Gut
Explant
Bacteria
Artiodactyla
Adhesion
Ungulata
Enterobacteriaceae
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Summary:We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization.
Bibliography:http://aem.asm.org/contents-by-date.0.shtml
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content type line 23
Corresponding author. Mailing address: Enteric Bacterial Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton Laboratory, Compton, Berkshire RG20 7NN, United Kingdom. Phone: 44 (0)1635 577915. Fax: 44 (0)1635 577237. E-mail: mark-p.stevens@bbsrc.ac.uk
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.02893-06