Development of immersion vaccine for bacterial cold-water disease in ayu Plecoglossus altivelis

• Published in: Bioscience, biotechnology, and biochemistry Vol. 81; no. 3; pp. 608 - 613
• Main Authors: Nakayama, Hitoshi, Mori, Mariko, Takita, Teisuke, Yasukawa, Kiyoshi, Tanaka, Keisuke, Hattori, Shunji, Aikawa, Hideaki, Hasegawa, Osamu, Okamura, Takashi, Takegami, Kentarou, Motokawa, Shogo, Kuwahara, Masakazu, Amano, Kenichi
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 04.03.2017; Oxford University Press
• Subjects: Animals; Aquaculture; ayu Plecoglossus altivelis; bacterial cold-water disease; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacterial Vaccines - genetics; Bacterial Vaccines - immunology; Bacterial Vaccines - pharmacology; Brevibacillus - genetics; collagenase; Collagenases - genetics; Collagenases - immunology; Escherichia coli - genetics; Fish Diseases - microbiology; Fish Diseases - prevention & control; Flavobacteriaceae Infections - prevention & control; Flavobacteriaceae Infections - veterinary; Flavobacterium - immunology; Flavobacterium - pathogenicity; Flavobacterium psychrophilum; immersion vaccine; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Vaccines, Synthetic - pharmacology; collagenase; immersion vaccine; ayu; bacterial cold-water disease; Flavobacterium psychrophilum; ayu Plecoglossus altivelis
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2016.1268041

Flavobacterium psychrophilum (F. psychrophilum) is the causative agent of bacterial cold-water disease (BCWD) that occurs in ayu Plecoglossus altivelis. Formalin-killed cell of F. psychrophilum has long been studied as an immersion vaccine for BCWD. In this study, we explored the possibility of F. psychrophilum collagenase (fpcol) for use as the immersion vaccine. BCWD convalescent ayu sera contained specific IgM antibodies against somatic F. psychrophilum and fpcol, meaning that fpcol is a promising antigen for the vaccine development. The recombinant fpcol was successfully expressed in Escherichia coli and Brevibacillus chosinensis (B. chosinensis). The culture supernatant of the B. chosinensis was used as an immersion vaccine solution. The vaccinated ayu were then challenged by soaking into F. psychrophilum culture. In two experimental groups, the relative percentages of survivals were 63 and 38%, respectively, suggesting that fpcol is promising as the immersion vaccine for ayu-BCWD.