Weissellicin 110, a Newly Discovered Bacteriocin from Weissella cibaria 110, Isolated from Plaa-Som, a Fermented Fish Product from Thailand

• Published in: Applied and Environmental Microbiology Vol. 73; no. 7; pp. 2247 - 2250
• Main Authors: Srionnual, Sirinat, Yanagida, Fujitoshi, Lin, Li-Hsiu, Hsiao, Kuang-Nan, Chen, Yi-sheng
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.04.2007
• Subjects: Amino Acid Sequence; Amino acids; Bacteria; Bacteriocins - chemistry; Bacteriocins - isolation & purification; Bacteriocins - pharmacology; Bacteriology; Biological and medical sciences; Fermentation; Fish; Fish Products - microbiology; Fundamental and applied biological sciences. Psychology; Genetics and Molecular Biology; Lactobacillus - metabolism; Microbiology; Molecular Sequence Data; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Thailand; Weissella; Thailand; Asia; Bacteria; Fermented product; Bacteriocin; Weissella
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.02484-06

Weissella cibaria 110, isolated from the Thai fermented fish product plaa-som, was found to produce a bacteriocin active against some gram-positive bacteria. Bacteriocin activity was not eliminated by exposure to high temperatures or catalase but was destroyed by exposure to the proteolytic enzymes proteinase K and trypsin. The bacteriocin from W. cibaria 110 was purified, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the purified bacteriocin contained one protein band that was approximately 2.5 kDa in size. Mass spectrometry analysis showed the mass of the peptide to be approximately 3,487.8 Da. N-terminal amino acid sequence analysis was performed, and 27 amino acids were identified. Because it has no similarity to other known bacteriocins, this bacteriocin was defined as a new bacteriocin and termed weissellicin 110.