A Label Free Electrochemical Nanobiosensor Study

• Published in: Analytical letters Vol. 42; no. 17; pp. 2905 - 2913
• Main Authors: Zhang, Juankun, Dong, Shanmu, Lu, Jinhui, Turner, Anthony P. F., Fan, Qingjie, Jia, Shiru, Yang, Hongjiang, Qiao, Changsheng, Zhou, Hao, He, Guowei
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01.01.2009; Taylor & Francis
• Subjects: Analytical chemistry; Biological and medical sciences; Biosensors; Biotechnology; Chemistry; Electrochemical methods; Electrolyte-insulator-semiconductor; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; impedance; Methods. Procedures. Technologies; nano-porous silicon; nanobiosensor; TECHNOLOGY; TEKNIKVETENSKAP; Various methods and equipments; Phosphates; Insulator; Electrochemical method; Immobilization; Laboratory; Signal; nanobiosensor; Electrolyte-insulator-semiconductor; Silicon; nano-porous silicon; Ungulata; Trace analysis; Bovine; Antibody; Serum albumin; Concentration; Buffer system; Chemical sensor; Antigen; Vertebrata; Sensitivity; Mammalia; Biosensor; Artiodactyla; Surface area; Impedance; Electrochemical impedance spectroscopy; impedance
• ISSN: 0003-2719; 1532-236X; 1532-236X
• DOI: 10.1080/00032710903201941

Nano-porous silicon (PS) is an attractive material for incorporation into biosensors, because it has a large surface area combined with the ability to generate both optical and electrical signals. In this paper, we describe a label-free nanobiosensor for bovine serum albumin (BSA). Nano-porous silicon produced in our laboratory was functionalized prior to immobilization of anti-BSA antibody on the surface. Reaction with BSA in phosphate buffered saline (PBS) buffer resulted in an impedance change which was inversely proportional to the concentration of the analyte. The system PBS buffer/antigen-antibody/PS constitutes an electrolyte-insulator-semiconductor (EIS) structure, thus furnishing an impedance EIS nanobiosensor. The linear range of the sensor was 0-0.27 mg mL −1 and the sensitivity was less than 10 µg mL −1 .