Selection of Sphingomonadaceae at the Base of Laccaria proxima and Russula exalbicans Fruiting Bodies

• Published in: Applied and Environmental Microbiology Vol. 75; no. 7; pp. 1979 - 1989
• Main Authors: Boersma, F.G. Hidde, Warmink, Jan A, Andreote, Fernando A, van Elsas, Jan Dirk
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.04.2009; American Society for Microbiology (ASM)
• Subjects: Bacteria; Basidiomycota; Biodiversity; Biological and medical sciences; Cluster Analysis; Colonization; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Electrophoresis, Polyacrylamide Gel - methods; Fruiting Bodies, Fungal; Fundamental and applied biological sciences. Psychology; Fungi; Laccaria; Microbiology; Molecular Sequence Data; Mycology; Phylogeny; Polymerase Chain Reaction - methods; Sequence Analysis, DNA; Sequence Homology; Soil Microbiology; Soil microorganisms; Soil sciences; Sphingomonadaceae - classification; Sphingomonadaceae - genetics; Sphingomonadaceae - isolation & purification; Fungi; Selection; Russula; Basidiomycota
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.02489-08

The dense hyphal network directly underneath the fruiting bodies of ectomycorrhizal fungi might exert strong influences on the bacterial community of soil. Such fruiting bodies might serve as hot spots for bacterial activity, for instance by providing nutrients and colonization sites in soil. Here, we assessed the putative selection of specific members of the Sphingomonadaceae family at the bases of the fruiting bodies of the ectomycorrhizal fungi Laccaria proxima and Russula exalbicans in comparison to the adjacent bulk soil. To do so, we used a previously designed Sphingomonadaceae-specific PCR-denaturing gradient gel electrophoresis (DGGE) system and complemented this with analyses of sequences from a Sphingomonadaceae-specific clone library. The analyses showed clear selective effects of the fruiting bodies of both fungi on the Sphingomonadaceae community structures. The effect was especially prevalent with R. exalbicans. Strikingly, similar fungi sampled approximately 100 m apart showed similar DGGE patterns, while corresponding bulk soil-derived patterns differed from each other. However, the mycospheres of L. proxima and R. exalbicans still revealed divergent community structures, indicating that different fungi select for different members of the Sphingomonadaceae family. Excision of specific bands from the DGGE patterns, as well as analyses of the clone libraries generated from both habitats, revealed fruiting body-specific Sphingomonadaceae types. It further showed that major groups from the mycospheres of R. exalbicans and L. proxima did not cluster with known bacteria from the database, indicating new groups within the family of Sphingomonadaceae present in these environments.