Lactobionic and cellobionic acid production profiles of the resting cells of acetic acid bacteria

• Published in: Bioscience, biotechnology, and biochemistry Vol. 79; no. 10; pp. 1712 - 1718
• Main Authors: Kiryu, Takaaki, Kiso, Taro, Nakano, Hirofumi, Murakami, Hiromi
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.10.2015; Oxford University Press
• Subjects: Acetic Acid - metabolism; acetic acid bacterium; Acetobacter - metabolism; cellobionic acid; Cellobiose - metabolism; Disaccharides - biosynthesis; Fermentation; Gluconobacter - metabolism; Gluconobacter frateurii; lactobionic acid; Lactose - metabolism; oxidation; Oxidation-Reduction; Substrate Specificity; cellobionic acid; oxidation; lactobionic acid; acetic acid bacterium; Gluconobacter frateurii
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2015.1038214

Lactobionic acid was produced by acetic acid bacteria to oxidize lactose. Gluconobacter spp. and Gluconacetobacter spp. showed higher lactose-oxidizing activities than Acetobacter spp. Gluconobacter frateurii NBRC3285 produced the highest amount of lactobionic acid per cell, among the strains tested. This bacterium assimilated neither lactose nor lactobionic acid. At high lactose concentration (30%), resting cells of the bacterium showed sufficient oxidizing activity for efficient production of lactobionic acid. These properties may contribute to industrial production of lactobionic acid by the bacterium. The bacterium showed higher oxidizing activity on cellobiose than that on lactose and produced cellobionic acid. Oxidation of lactose and cellobiose by acetic acid bacteria produced lactobionic and cellobionic acid, respectively. Lactobionic acid was effectively produced under high-lactose conditions.