Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii

• Published in: Journal of AOAC International Vol. 92; no. 2; pp. 375 - 381
• Main Authors: Islam, M. Nurul, Yoo, Hye Hyun, Sung, Chung-Ki, Dong, Mi-Sook, Park, Young In, Jin, Changbae, Kim, Dong-Hyun
• Format: Journal Article
• Language: English
• Published: Gaithersburg, MD AOAC International 01.03.2009; Oxford University Press
• Subjects: 4-Butyrolactone - analogs & derivatives; 4-Butyrolactone - analysis; Araceae; Benzofurans - analysis; Benzofurans - standards; Biological and medical sciences; chemical constituents of plants; Chemical properties; chlorogenic acid; Chlorogenic Acid - analysis; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; Cnidium; Cnidium - chemistry; Cnidium officinale; Coumaric Acids - analysis; dietary supplements; dried herbs; ethanol; ferulic acid; Food industries; Fundamental and applied biological sciences. Psychology; herbal medicines; Hydroxybenzoates - analysis; Hydroxybenzoates - standards; Identification and classification; Korea; Liquid chromatography; Measurement; Medicinal plants; Medicine, Korean Traditional; Methods; Oriental traditional medicine; phenolic acids; Phenols; phthalides; phytochemicals; plant extracts; Plant Extracts - analysis; Plant Extracts - standards; Quality Control; quantitative analysis; Reproducibility of Results; reversed-phase high performance liquid chromatography; rhizomes; Spectrometry, Mass, Electrospray Ionization; supercritical fluid extraction; South Korea; Japan; China; United States; Phenolic acid; Quality; Liquid chromatography
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/92.2.375

This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C18 chromatographic column (150 x 4.6 mm, 3 μm) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r2 >0.99) was observed over the concentration range of 2-100 μg/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were <or=5.37% and accuracies ranged from 97.1 to 104.9%. Recoveries of the compounds ranged from 94.2 to 104.2% with RSD values of <=9.50%. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.84-5.35, 0.45-1.65, 0.74-4.39, and 0.32-1.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds.