HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction

• Published in: Physiological genomics Vol. 50; no. 7; pp. 479 - 494
• Main Authors: Williams, Allison Lesher, Khadka, Vedbar, Tang, Mingxin, Avelar, Abigail, Schunke, Kathryn J, Menor, Mark, Shohet, Ralph V
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.07.2018
• Series: Genomic and “Polyomic” Studies of Cardiovascular and Inflammatory Diseases
• Subjects: Alternative Splicing; Angiogenesis; Animals; Cardiac muscle; Chromatin; Gene expression; Gene Expression Profiling; Glycolysis; Glycolysis - genetics; Heart attacks; Humans; Hypoxia; Hypoxia-inducible factor 1; Hypoxia-Inducible Factor 1 - genetics; Hypoxia-Inducible Factor 1 - metabolism; Immunoprecipitation; Ischemia; Isoenzymes - genetics; Isoenzymes - metabolism; Isoforms; Kinases; Male; Metabolism; Mice, Inbred C57BL; Muscle contraction; Myocardial infarction; Myocardial Infarction - genetics; Myocardial Infarction - metabolism; Myocardial Infarction - physiopathology; PkM gene; Pyruvate kinase; Pyruvate Kinase - genetics; Pyruvate Kinase - metabolism; Pyruvic acid; Ribonucleic acid; RNA; Splicing factors; RNA sequencing; hypoxia; alternative splicing; myocardial infarction; pyruvate kinase
• ISSN: 1094-8341; 1531-2267
• DOI: 10.1152/physiolgenomics.00130.2017

Alternative splicing of RNA is an underexplored area of transcriptional response. We expect that early changes in alternatively spliced genes may be important for responses to cardiac injury. Hypoxia inducible factor 1 (HIF1) is a key transcription factor that rapidly responds to loss of oxygen through alteration of metabolism and angiogenesis. The goal of this study was to investigate the transcriptional response after myocardial infarction (MI) and to identify novel, hypoxia-driven changes, including alternative splicing. After ligation of the left anterior descending artery in mice, we observed an abrupt loss of cardiac contractility and upregulation of hypoxic signaling. We then performed RNA sequencing on ischemic heart tissue 1 and 3 days after infarct to assess early transcriptional changes and identified 89 transcripts with altered splicing. Of particular interest was the switch in Pkm isoform expression (pyruvate kinase, muscle). The usually predominant Pkm1 isoform was less abundant in ischemic hearts, while Pkm2 and associated splicing factors (hnRNPA1, hnRNPA2B1, Ptbp1) rapidly increased. Despite increased Pkm2 expression, total pyruvate kinase activity remained reduced in ischemic myocardial tissue. We also demonstrated HIF1 binding to PKM by chromatin immunoprecipitation, indicating a direct role for HIF1 in mediating this isoform switch. Our study provides a new, detailed characterization of the early transcriptome after MI. From this analysis, we identified an HIF1-mediated alternative splicing event in the PKM gene. Pkm1 and Pkm2 play distinct roles in glycolytic metabolism and the upregulation of Pkm2 is likely to have important consequences for ATP synthesis in infarcted cardiac muscle.