Zinc Content of Blood Cellular Components and Lymph Node and Spleen Lymphocytes in Severely Zinc-Deficient Rats
Several investigators have suggested that the measurement of leukocyte zinc may be useful for the assessment of zinc nutriture. However, in an earlier study, we found that the methods used did not adequately separate platelets from the different leukocyte populations. We subsequently developed a met...
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Published in | The Journal of nutrition Vol. 115; no. 8; pp. 1073 - 1078 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
Elsevier Inc
01.08.1985
American Society for Nutritional Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Several investigators have suggested that the measurement of leukocyte zinc may be useful for the assessment of zinc nutriture. However, in an earlier study, we found that the methods used did not adequately separate platelets from the different leukocyte populations. We subsequently developed a method which does adequately separate platelets, mononucleated cells (MNC), polymorphonucleated cells (PMN), and erythrocytes (RBC) from a single blood sample. This method was applied to a study of severe zinc deficiency in adult male rats. Significant reductions in plasma and femur zinc indicated severe zinc deficiency in the zinc-deficient animals compared with pair-fed or ad libitum-fed control rats in two separate experiments. However, we noted no differences in the zinc content of the MNC, PMN, RBC or lymph node lymphocytes, on a per cell basis, between the zinc-deficient and ad libitum- or pair-fed control groups of rats. A minor, but not significant (P > 0.05) reduction of platelet zinc was noted in zinc-deficient rats in two separate studies. These data indicate that the zinc contents of blood cellular components are not sensitive indicators of zinc deficiency in rats. |
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Bibliography: | 8608249 S30 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-3166 1541-6100 |
DOI: | 10.1093/jn/115.8.1073 |